First molecular detection of porcine circovirus type 2 in bovids in China

• Published in: Virus genes Vol. 49; no. 3; pp. 507 - 511
• Main Authors: Zhai, Shao-Lun, Chen, Rui-Ai, Chen, Sheng-Nan, Wen, Xiao-Hui, Lv, Dian-Hong, Wu, Da-Cheng, Yuan, Jie, Huang, Zhong, Zhou, Xiu-Rong, Luo, Man-Lin, He, Dong-Sheng, Wei, Wen-Kang
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.12.2014; Springer US; Springer Nature B.V
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; blood serum; buffaloes; Cattle; Cattle Diseases - epidemiology; Cattle Diseases - virology; China - epidemiology; Circoviridae Infections - epidemiology; Circoviridae Infections - veterinary; Circoviridae Infections - virology; Circovirus - classification; Circovirus - genetics; Circovirus - isolation & purification; Cluster Analysis; dairy cows; DNA, Viral - chemistry; DNA, Viral - genetics; Genetic Variation; Genotype; Medical Microbiology; Molecular Sequence Data; muscles; Muscles - virology; pathogens; Phylogeny; Plant Sciences; Polymerase Chain Reaction; Porcine circovirus; Porcine circovirus-2; Sequence Analysis, DNA; Sequence Homology; Serum - virology; Virology; China; Porcine circovirus type 2; Co-existence; Genetic diversity; Molecular detection; Bovids
• ISSN: 0920-8569; 1572-994X
• DOI: 10.1007/s11262-014-1117-1

For the worldwide pig industries, porcine circovirus type 2 (PCV2) is an economically important pathogen. At present, the prevalence of PCV2 is common in Chinese swine herds. However, there is little information on PCV2 prevalence in non-porcine animals in China, such as bovids. Therefore, the goal of this study is to obtain the firsthand prevalence data of PCV2 in bovids in China. Two hundred and eighty serum and muscle samples from dairy cows (n = 180), buffalo (n = 50), and yellow cattle (n = 50) were analyzed by PCR. The detection results show that PCV2 infections (16 %, 8/50) only exist in buffaloes. In addition, there are different PCV2 viral DNAs identified by differential PCR in the same buffalo sample. Nucleotide sequencing and phylogenetic analysis results based on partial ORF1 and ORF2 sequences suggest that PCV2 strains have genetic diversity in buffaloes and they are divided into three different genotypes (PCV2b, PCV2d, and PCV2e, respectively). Moreover, to our knowledge, the PCV2d and PCV2e genotypes have not been previously reported in bovids. Through this study, the firsthand data of PCV2 prevalence in bovids in China was documented.