Genomics reveals introgression and purging of deleterious mutations in the Arabian leopard (Panthera pardus nimr)

• Published in: iScience Vol. 26; no. 9; p. 107481
• Main Authors: Mochales-Riaño, Gabriel, Fontsere, Claudia, de Manuel, Marc, Talavera, Adrián, Burriel-Carranza, Bernat, Tejero-Cicuéndez, Héctor, AlGethami, Raed Hamoud M., Shobrak, Mohammed, Marques-Bonet, Tomas, Carranza, Salvador
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 15.09.2023; Elsevier
• Subjects: Genomics; Zoology; Genomics; Zoology
• ISSN: 2589-0042; 2589-0042
• DOI: 10.1016/j.isci.2023.107481

In endangered species, low-genetic variation and inbreeding result from recent population declines. Genetic screenings in endangered populations help to assess their vulnerability to extinction and to create informed management actions toward their conservation efforts. The leopard, Panthera pardus, is a highly generalist predator with currently eight different subspecies. Yet, genomic data are still lacking for the Critically Endangered Arabian leopard (P. p. nimr). Here, we sequenced the whole genome of two Arabian leopards and assembled the most complete genomic dataset for leopards to date. Our phylogenomic analyses show that leopards are divided into two deeply divergent clades: the African and the Asian. Conservation genomic analyses indicate a prolonged population decline, which has led to an increase in inbreeding and runs of homozygosity, with consequent purging of deleterious mutations in both Arabian individuals. Our study represents the first attempt to genetically inform captive breeding programmes for this Critically Endangered subspecies. [Display omitted] •Two new whole genomes are generated for the Critically Endangered Arabian leopard•Phylogenomics support Arabian leopards as a distinct lineage sister to Asian leopards•Arabian leopard has suffered a prolonged bottleneck•High inbreeding and ROH but purging of deleterious mutations in Arabian leopards Genomics; Zoology; Leopards