Female and male obese Zucker rats display differential inflammatory mediator and long non-coding RNA profiles

The goal of this study was to identify mediators in peri-lymphatic adipose tissue (PLAT) that are altered in obese versus lean Zucker rats, with focus on potential sex differences MAIN METHODS: Mesenteric PLAT was analyzed with protein and lncRNA arrays. Additional RT-PCR confirmation was performed...

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Published inLife sciences (1973) Vol. 335; p. 122285
Main Authors Patel, Niketa A., Lui, Ashley, Trujillo, Andrea N., Motawe, Zeinab Y., Bader, Deena, Schuster, Jane, Burgess, Andrea, Alves, Natascha G., Jo, Michiko, Breslin, Jerome W.
Format Journal Article
LanguageEnglish
Published Netherlands 15.12.2023
Subjects
adipose tissue
Adipose Tissue - metabolism
Animals
epididymis
Female
females
galectins
histology
Humans
inflammation
Inflammation - genetics
Inflammation - metabolism
Inflammation Mediators
landscapes
macrophages
Male
males
non-coding RNA
obesity
Obesity - genetics
Obesity - metabolism
proteome
Rats
Rats, Zucker
RNA, Long Noncoding - genetics
therapeutics
Obesity
Adipose
Inflammation
Metabolic syndrome
Long non-coding RNA
Online AccessGet full text
ISSN0024-3205
1879-0631
1879-0631
DOI10.1016/j.lfs.2023.122285

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Summary:The goal of this study was to identify mediators in peri-lymphatic adipose tissue (PLAT) that are altered in obese versus lean Zucker rats, with focus on potential sex differences MAIN METHODS: Mesenteric PLAT was analyzed with protein and lncRNA arrays. Additional RT-PCR confirmation was performed with epididymal/ovarian fat. MCP-1, TCK-1, Galectin-1, Galectin-3, and neuropilin-1 were elevated in PLAT from obese rats of both sexes. However, 11 additional proteins were elevated only in obese males while 24 different proteins were elevated in obese females. Profiling of lncRNAs revealed lean males have elevated levels of NEAT1, MALAT1 and GAS5 compared to lean females. NEAT1, MALAT1, and GAS5 were significantly reduced with obesity in males but not in females. Another lncRNA, HOTAIR, was higher in lean females compared to males, and its levels in females were reduced with obesity. Obese rats of both sexes had similar histologic findings of mesenteric macrophage crown-like structures and hepatocyte fat accumulation. While obese male and female Zucker rats both have increased inflammation, they have distinct signals. Future studies of the proteome and lncRNA landscape of obese males vs. females in various animal models and in human subjects are warranted to better guide development of therapeutics for obesity-induced inflammation.
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AUTHOR CONTRIBUTIONS
Conceptualization, N.A.P., A.N.T., and J.W.B.; data curation, N.A.P., A.L., A.N.T., J.S, J.W.B.; formal analysis, N.A.P., A.L, A.N.T., J.W.B..; funding acquisition, N.A.P., J.S., M.J., J.W.B.; investigation, A.L., A.N.T., Z.Y.M., D.B., J.S., N.G.A., M.J.; methodology, N.A.P., A.L., A.N.T., N.G.A., J.W.B..; project administration, N.A.P, J.W.B.; resources, N.A.P., J.W.B.; software, J.W.B.; supervision, N.A.P., A.N.T., J.W.B.; validation, A.L, A.N.T., D.B, J.S., Z.Y.M.; visualization, A.N.T., Z.Y.M., J.S.; writing—original draft preparation, N.A.P., A.L., A.N.T., J.W.B.; writing—review and editing, Z.Y.M., J.S., N.G.A..; All authors have read and agreed to the published version of the manuscript.
ISSN:0024-3205
1879-0631
1879-0631
DOI:10.1016/j.lfs.2023.122285