Genomic and Phenotypic Characterization of Cutibacterium acnes Bacteriophages Isolated from Acne Patients

Cutibacterium acnes is a pathogen that can cause acne vulgaris, sarcoidosis, endodontic lesions, eye infections, prosthetic joint infections, and prostate cancer. Recently, bacteriophage (phage) therapy has been developed as an alternative to antibiotics. In this study, we attempted to isolate 15 ph...

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Published inAntibiotics (Basel) Vol. 11; no. 8; p. 1041
Main Authors Kim, Shukho, Song, Hyesoon, Jin, Jong Sook, Lee, Weon Ju, Kim, Jungmin
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 02.08.2022
MDPI
Subjects
Acne
Adsorption
Amino acids
Antibiotics
Antimicrobial agents
Bacteria
bacteriophage
Cutibacterium acnes
Drug resistance
E coli
Genomes
Hydrophobicity
Infections
Patients
phage therapy
Phages
Prostate cancer
Prostheses
Proteins
Sarcoidosis
Staphylococcus infections
Virulence
hydrophobicity
Cutibacterium acnes
bacteriophage
phage therapy
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Summary:Cutibacterium acnes is a pathogen that can cause acne vulgaris, sarcoidosis, endodontic lesions, eye infections, prosthetic joint infections, and prostate cancer. Recently, bacteriophage (phage) therapy has been developed as an alternative to antibiotics. In this study, we attempted to isolate 15 phages specific to C. acnes from 64 clinical samples obtained from patients with acne vulgaris. Furthermore, we sequenced the genomes of these three phages. Bioinformatic analysis revealed that the capsid and tape measure proteins are strongly hydrophobic. To efficiently solubilize the phage particles, we measured the adsorption rate, one-step growth curve, and phage stability using an SMT2 buffer containing Tween 20. Here, we report the genotypic and phenotypic characteristics of the novel C. acnes-specific phages.
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Present address: Center for FMD Vaccine Research, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics11081041