Structural Basis of HIV-1 Tethering to Membranes by the BST-2/Tetherin Ectodomain

• Published in: Cell host & microbe Vol. 7; no. 4; pp. 314 - 323
• Main Authors: Hinz, Andreas, Miguet, Nolwenn, Natrajan, Ganesh, Usami, Yoshiko, Yamanaka, Hikaru, Renesto, Patricia, Hartlieb, Bettina, McCarthy, Andrew A., Simorre, Jean-Pierre, Göttlinger, Heinrich, Weissenhorn, Winfried
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 22.04.2010; Elsevier
• Subjects: Animals; Antigens, CD - chemistry; Antigens, CD - metabolism; Bacteriology; Biochemistry, Molecular Biology; Cell Membrane - virology; CELLBIO; Circular Dichroism; Crystallography, X-Ray; GPI-Linked Proteins; HIV-1 - physiology; Host-Pathogen Interactions; Life Sciences; Membrane Glycoproteins - chemistry; Membrane Glycoproteins - metabolism; MICROBIO; Microbiology and Parasitology; Models, Molecular; Mutagenesis, Site-Directed; Protein Stability; Protein Structure, Secondary; Protein Structure, Tertiary; Scattering, Small Angle; Structural Biology; Virology; Virus Release; CELLBIO; MICROBIO
• ISSN: 1931-3128; 1934-6069
• DOI: 10.1016/j.chom.2010.03.005

The restriction factor BST-2/tetherin contains two membrane anchors employed to retain some enveloped viruses, including HIV-1 tethered to the plasma membrane in the absence of virus-encoded antagonists. The 2.77 Å crystal structure of the BST-2/tetherin extracellular core presented here reveals a parallel 90 Å long disulfide-linked coiled-coil domain, while the complete extracellular domain forms an extended 170 Å long rod-like structure based on small-angle X-ray scattering data. Mutagenesis analyses indicate that both the coiled coil and the N-terminal region are required for retention of HIV-1, suggesting that the elongated structure can function as a molecular ruler to bridge long distances. The structure reveals substantial irregularities and instabilities throughout the coiled coil, which contribute to its low stability in the absence of disulfide bonds. We propose that the irregular coiled coil provides conformational flexibility, ensuring that BST-2/tetherin anchoring both in the plasma membrane and in the newly formed virus membrane is maintained during virus budding. ► The BST-2/tetherin ectodomain forms a dimeric 170 Å long bent rod-like structure ► The ectodomain includes a 90 Å long parallel coiled coil ► Both the N-terminal region and the coiled coil are required for HIV-1 retention ► Irregular features of the coiled coil ensure flexibility during budding