Infrared Multiple Photon Dissociation Action Spectroscopy and Theoretical Studies of Diethyl Phosphate Complexes: Effects of Protonation and Sodium Cationization on Structure
The gas-phase structures of deprotonated, protonated, and sodium-cationized complexes of diethyl phosphate (DEP) including [DEP − H] − , [DEP + H] + , [DEP + Na] + , and [DEP − H + 2Na] + are examined via infrared multiple photon dissociation (IRMPD) action spectroscopy using tunable IR radiation ge...
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Published in | Journal of the American Society for Mass Spectrometry Vol. 22; no. 1; pp. 81 - 92 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer-Verlag
2011
Elsevier Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | The gas-phase structures of deprotonated, protonated, and sodium-cationized complexes of diethyl phosphate (DEP) including [DEP − H]
−
, [DEP + H]
+
, [DEP + Na]
+
, and [DEP − H + 2Na]
+
are examined via infrared multiple photon dissociation (IRMPD) action spectroscopy using tunable IR radiation generated by a free electron laser, a Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) with an electrospray ionization (ESI) source, and theoretical electronic structure calculations. Measured IRMPD spectra are compared to linear IR spectra calculated at the B3LYP/6-31G(d,p) level of theory to identify the structures accessed in the experimental studies. For comparison, theoretical studies of neutral complexes are also performed. These experiments and calculations suggest that specific geometric changes occur upon the binding of protons and/or sodium cations, including changes correlating to nucleic acid backbone geometry, specifically P–O bond lengths and ∠OPO bond angles. Information from these observations may be used to gain insight into the structures of more complex systems, such as nucleotides and solvated nucleic acids. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1044-0305 1879-1123 |
DOI: | 10.1007/s13361-010-0007-6 |