Staphylococcus aureus in Horses in Nigeria: Occurrence, Antimicrobial, Methicillin and Heavy Metal Resistance and Virulence Potentials

• Published in: Antibiotics (Basel) Vol. 12; no. 2; p. 242
• Main Authors: Nwobi, Obichukwu Chisom, Anyanwu, Madubuike Umunna, Jaja, Ishmael Festus, Nwankwo, Innocent Okwundu, Okolo, Chukwuemeka Calistus, Nwobi, Chibundo Adaobi, Ezenduka, Ekene Vivienne, Oguttu, James Wabwire
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24.01.2023; MDPI
• Subjects: Agglutination; Antibiotic resistance; Antimicrobial resistance; Biofilms; Cadmium; Catalase; Clindamycin; Dilution; Drug resistance; equine; Gelatinase; heavy metal tolerance; Heavy metals; Horses; Latex; Latex agglutination; Methicillin; methicillin resistance; Multidrug resistance; Penicillin; Staphylococcus aureus; Staphylococcus infections; Tellurite; Tellurium dioxide; Vancomycin; Virulence; Yolk; β Lactamase; England; Hampshire England; United Kingdom > UK; Nigeria; methicillin resistance; heavy metal tolerance; virulence; Staphylococcus aureus; equine
• ISSN: 2079-6382; 2079-6382
• DOI: 10.3390/antibiotics12020242

was isolated from a total of 360 nasal and groin skin swabs from 180 systematic randomly-selected horses slaughtered for meat at Obollo-Afor, Enugu State, Southeast Nigeria and antimicrobial, methicillin and heavy metal resistance profile and virulence potentials of the isolates established. Baird-Parker agar with egg yolk tellurite was used for . isolation. . isolates were confirmed biochemically and serologically using a specific . Staphytect Plus™ latex agglutination test kit. The antimicrobial resistance profile, methicillin, vancomycin and inducible clindamycin resistance, and β-lactamase production of the isolates were determined with disc diffusion. Tolerance to Copper, Cadmium, Lead and Zinc was assessed using the agar dilution method and virulence potentials were determined using phenotypic methods. Forty-three (23.9%) of the 180 horses harbored . . Some 71 . were recovered from the 360 samples. Two (2.8%) of the 71 . were methicillin-resistant . (MRSA) and 69 (97.2%) were methicillin-susceptible. MRSA was recovered from 2 (1.1%) of the 180 horses. Some 9.4% of the isolates were multiple drug-resistant (MDR). The mean multiple antibiotic resistance indices (MARI) for the isolates was 0.24. Heavy metal resistance rate of the isolates ranged between 35.4-70.4%. The isolates, including the MRSA strains, displayed virulence potentials as clumping factor and catalase, gelatinase, caseinase, heamolysin, and biofilm was at the rate of 100%, 53.5%, 43.7%, 18.3% and 23.9%, respectively. This study showed that a considerable percentage of horses slaughtered in Obollo-Afor Southeastern Nigeria are potential reservoirs of virulent multiple drug- and heavy metal-resistant . , including MRSA, that could spread to humans and the environment.