Knockdown of IGF-IR by siRNA injection during bovine preimplantation embryonic development

• Published in: Cytotechnology (Dordrecht) Vol. 64; no. 2; pp. 165 - 172
• Main Authors: Wang, L. M., Wen, J. X., Yuan, J. L., Cang, Ming, Liu, D. J.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.03.2012; Springer; Springer Nature B.V
• Subjects: Antibodies; Biochemistry; Biological and medical sciences; Biomedicine; Biotechnology; Blastocysts; Cell number; Chemistry; Chemistry and Materials Science; Efficiency; Embryogenesis; Embryos; Fundamental and applied biological sciences. Psychology; In vitro fertilization; Infrared imaging systems; Insulin-like growth factor I receptors; Insulin-like growth factors; Microinjection; mRNA; Oocytes; Original Research; siRNA; Variance analysis; Zygotes; United States > US; IGF; RNAi; Preimplantation embryo; Bovine; In vitro; Embryonic development; Embryo; RNA interference; siRNA; Gene silencing; Vertebrata; Mammalia; Artiodactyla; Ungulata
• ISSN: 0920-9069; 1573-0778
• DOI: 10.1007/s10616-011-9402-5

This study aimed to assess the efficiency and effects of insulin-like growth factor receptor-1 (IGF-IR) siRNA knockdown during bovine preimplantation embryonic development. In oocytes injected with IGF-IR siRNA, the relative IGF-IR mRNA levels compared to controls were 28% and 46% at 6 and 24 h after injection, respectively. With respect to the injection of IGF-IR siRNA in zygotes, 24 h after injection the relative levels of IGF-IR mRNA and protein in the two-cell embryos were 74% and 78% of those in the controls, respectively. IGF-IR siRNA reduced blastocyst formation (23.2%) compared to siRNA controls (33.0%) and uninjected oocytes (35.4%; P  < 0.05) and the number of viable cells per IGF-IR siRNA-treated blastocyst (64 ± 3) was significantly reduced, compared to control siRNA and uninjected blastocysts (81 ± 3 and 116 ± 4; P  < 0.01). In conclusion, IGF-IR siRNA knockdown reduces the development of bovine embryos, and microinjection in zygotes can decrease blastocyst cell number.