Development and verification of new reference plasmid (pUC_GM-SB) for the DNA-based detection of genetically modified sugar beet H7-1

• Published in: Food science and biotechnology Vol. 33; no. 14; pp. 3291 - 3297
• Main Authors: Suh, Seung-Man, Kim, Sumin, Kim, Hyun-Jae, Shin, Min-Ki, Kim, Hae-Yeong
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 01.11.2024; Springer Nature B.V; 한국식품과학회
• Subjects: Chemistry; Chemistry and Materials Science; detection limit; Food safety; Food Science; genes; Genetic modification; herbicide resistance; Herbicides; market share; Nutrition; plasmids; Polymerase chain reaction; quantitative polymerase chain reaction; Research Article; Standard deviation; Sugar; sugar beet; Sugar beets; 식품과학; Reference plasmid; GM sugar beet; Quantitative PCR; Event-specific primer; Qualitative PCR
• ISSN: 1226-7708; 2092-6456; 2092-6456
• DOI: 10.1007/s10068-024-01572-6

The sugar beet is the second-largest sugar-producing crop. Genetically modified (GM) sugar beet, which have herbicide-resistant, have been developed to increase production and comprise over 90% of the market share. This study describes qualitative and quantitative PCR methods for the GM sugar beet H7-1 with reference plasmid (pUC_GM-SB) containing an endogenous gene ( GS2 ) and an event-specific gene for H7-1 that served as a positive control for PCR. The detection limit of qualitative PCR was approximately 10 copies of the reference plasmid and 0.05% in spiked samples. In the case of quantitative PCR, the detection limit was five copies of the reference plasmid. Regarding repeatability, the standard deviation and relative standard deviation were found to range from 0.11 to 0.24 and from 0.23% to 0.99%, respectively. This study provides food safety assurance for imported GM sugar beet H7-1 using the reference plasmid and supports efficient detection methods.