Purification and mode of action of two different arabinoxylan arabinofuranohydrolases from Bifidobacterium adolescentis DSM 20083

• Published in: Applied microbiology and biotechnology Vol. 51; no. 5; pp. 606 - 613
• Main Authors: Laere, K.M.J. van, Voragen, C.H.L, Kroef, T, Broek, L.A.M. van, Beldman, G, Voragen, A.G.J
• Format: Journal Article
• Language: English
• Published: Berlin Springer 1999; Springer Nature B.V
• Subjects: Aspergillus awamori; Bacteria; Bifidobacterium adolescentis; Biological and medical sciences; Biological Sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; Food Chemistry and Microbiology; food microbiology; food processing; Fundamental and applied biological sciences. Psychology; Levensmiddelenchemie en -microbiologie; Miscellaneous; Mission oriented research; Mode of action; Residues; VLAG; Actinomycetales; Purification; Actinomycetaceae; Enzyme; Bifidobacterium adolescentis; Bacteria; Hydrolases; Actinomycetes; Kinetic parameter; Mechanism of action; Comparative study; Physicochemical properties
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s002530051439

Two novel arabinofuranohydrolases (AXH-d3 and AXH-m23) were purified from Bifidobacterium adolescentis DSM 20083. Both enzymes were induced upon growth of Bi. adolescentis on xylose and arabinoxylan-derived oligosaccharides. They were only active with arabinoxylans and therefore denoted as arabinoxylan arabinofuranohydrolases. Their optimal activity was at pH 6 and 30-40 degrees C. They were very specific in their mode of action and were clearly different from AXH-m from Aspergillus awamori. AXH-m23 released only arabinosyl groups, which were linked to the C-2 or C-3 position of singly substituted xylose residues in arabinoxylan oligomers. AXH-d3 hydrolysed C-3-linked arabinofuranosyl residues of doubly substituted xylopyranosyl residues of arabinoxylans and arabinoxylan-derived oligosaccharides. No activity was observed with C-2-linked arabinofuranosyl residues of these doubly substituted xylopyranosyl residues, or against C-2- and C-3-linked arabinofuranosyl residues of singly substituted xylopyranosyl residues. The combination of AXH-d3 and AXH-m showed low debranching activity with highly substituted glucuronoarabinoxylans. However, arabinoxylan from wheat flour was debranched almost completely.