Hydrogenophaga electricum sp. nov., isolated from anodic biofilms of an acetate-fed microbial fuel cell

A Gram-negative, non-spore-forming, rod-shaped bacterial strain, AR20T, was isolated from anodic biofilms of an acetate-fed microbial fuel cell in Japan and subjected to a polyphasic taxonomic study. Strain AR20T grew optimally at pH 7.0−8.0 and 25°C. It contained Q-8 as the predominant ubiquinone a...

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Bibliographic Details
Published inJournal of general and applied microbiology Vol. 59; no. 4; pp. 261 - 266
Main Authors Kimura, Zen-ichiro, Okabe, Satoshi
Format Journal Article
LanguageEnglish
Published Japan Applied Microbiology, Molecular and Cellular Biosciences Research Foundation 01.01.2013
Japan Science and Technology Agency
Subjects
Acetates - metabolism
Base Composition
Bioelectric Energy Sources - microbiology
Biofilms - growth & development
Cluster Analysis
Comamonadaceae - classification
Comamonadaceae - genetics
Comamonadaceae - isolation & purification
Comamonadaceae - physiology
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Electrodes - microbiology
Fatty Acids - analysis
Hydrogen-Ion Concentration
Hydrogenophaga electricum
hydrogenotrophic exoelectrogen
Japan
microbial fuel cell
Microscopy
Molecular Sequence Data
Phylogeny
Quinones - analysis
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Temperature
Japan
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Summary:A Gram-negative, non-spore-forming, rod-shaped bacterial strain, AR20T, was isolated from anodic biofilms of an acetate-fed microbial fuel cell in Japan and subjected to a polyphasic taxonomic study. Strain AR20T grew optimally at pH 7.0−8.0 and 25°C. It contained Q-8 as the predominant ubiquinone and C16:0, summed feature 3 (C16:1ω7c and/or iso-C15:02OH), and C18:1ω7c as the major fatty acids. The DNA G+C content was 67.1 mol%. A neighbor-joining phylogenetic tree revealed that strain AR20T clustered with three type strains of the genus Hydrogenophaga (H. flava, H. bisanensis and H. pseudoflava). Strain AR20T exhibited 16S rRNA gene sequence similarity values of 95.8−97.7% to the type strains of the genus Hydrogenophaga. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain AR20T is considered a novel species of the genus Hydrogenophaga, for which the name Hydrogenophaga electricum sp. nov. is proposed. The type strain is AR20T (= KCTC 32195T = NBRC 109341T).
ISSN:0022-1260
1349-8037
DOI:10.2323/jgam.59.261