Enzymatic fingerprinting reveals specific xyloglucan and pectin signatures in the cell wall purified with primary plasmodesmata
Plasmodesmata (PD) pores connect neighbouring plant cells and enable direct transport across the cell wall. Understanding the molecular composition of these structures is essential to address their formation and later dynamic regulation. Here we provide a biochemical characterisation of the cell wal...
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Published in | Frontiers in plant science Vol. 13; p. 1020506 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers
25.10.2022
Frontiers Media S.A |
Subjects | |
Online Access | Get full text |
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Summary: | Plasmodesmata (PD) pores connect neighbouring plant cells and enable direct transport across the cell wall. Understanding the molecular composition of these structures is essential to address their formation and later dynamic regulation. Here we provide a biochemical characterisation of the cell wall co-purified with primary PD of
cell cultures. To achieve this result we combined subcellular fractionation, polysaccharide analyses and enzymatic fingerprinting approaches. Relative to the rest of the cell wall, specific patterns were observed in the PD fraction. Most xyloglucans, although possibly not abundant as a group, were fucosylated. Homogalacturonans displayed short methylated stretches while rhamnogalacturonan I species were remarkably abundant. Full rhamnogalacturonan II forms, highly methyl-acetylated, were also present. We additionally showed that these domains, compared to the broad wall, are less affected by wall modifying activities during a time interval of days. Overall, the protocol and the data presented here open new opportunities for the study of wall polysaccharides associated with PD. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Vincenzo Lionetti, Sapienza University of Rome, Italy; Yoselin Benitez-Alfonso, University of Leeds, United Kingdom These authors share last authorship This article was submitted to Plant Physiology, a section of the journal Frontiers in Plant Science Edited by: Igor Cesarino, University of São Paulo, Brazil ORCID: A. Paterlini, orcid.org/0000-0002-1777-3160; J. Sechet, orcid.org/0000-0001-8398-4743; F. Immel, orcid.org/0000-0002-2028-902X; M. S. Grison, orcid.org/0000-0002-3080-3686; J. Pelloux, orcid.org/0000-0002-9371-1711; G. Mouille, orcid.org/0000-0002-5493-754X; E. M. Bayer, orcid.org/0000-0001-8642-5293; A. Voxeur, orcid.org/0000-0001-9452-6756 |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2022.1020506 |