Differential expression profiling of hypothalamus genes in laying period and ceased period Huoyan geese

• Published in: Molecular biology reports Vol. 41; no. 5; pp. 3401 - 3411
• Main Authors: Luan, Xinhong, Cao, Zhongzan, Li, Rongrong, Liu, Mei, Hu, Jianmin
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.05.2014; Springer Netherlands; Springer Nature B.V
• Subjects: Animal Anatomy; Animal Biochemistry; Animal populations; Animals; bioinformatics; Biomedical and Life Sciences; Brain; cell adhesion; cell differentiation; Computational Biology; Eggs; Expressed Sequence Tags; Female; geese; Geese - genetics; Gene expression; Gene Expression Profiling; Gene Expression Regulation; Gene Library; genes; gonadotropin-releasing hormone; Histology; Hybridization; hypothalamus; Hypothalamus - metabolism; laying performance; Life Sciences; Morphology; nitrogen; oviposition; quantitative analysis; Reproducibility of Results; Reproduction - genetics; reverse transcriptase polymerase chain reaction; secretion; signal transduction; suppression subtractive hybridization; Wildfowl; Hypothalamus; Suppression subtractive hybridization (SSH); Geese; Differential expression genes; Egg production
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-014-3202-x

The hypothalamus plays a central role in controlling poultry reproductive activity. To increase our understanding of genes involved in egg laying of Huoyan geese, gene profiles in the hypothalamus of laying period and ceased period Huoyan geese were investigated using suppression subtractive hybridization (SSH) method. A total of 95 differentially expressed sequence tags (ESTs), including 46 up-regulated and 49 down-regulated sequences showed homology to known genes of the non-redundant NCBI databases. Bioinformatic analysis demonstrated that these genes were mainly involved in anatomical structure development, signal transduction, cellular nitrogen compound metabolic process, biosynthetic process, cellular protein modification process, cell differentiation, transport, cell adhesion, and reproduction. Ten ESTs were selected for further analyses by quantitative real-time RT-PCR (qRT-PCR). Whose most part of results were consistent with the SSH results. Of note, AdipoR2, Nrg1, and NCAM1, which related with secretion of GnRH and other hormones, were identified to be differentially expressed between laying period and ceased period. These findings provided a new source for mining genes related to higher laying performance of Huoyan geese, which facilitate our understanding of the reproductive biology of the goose.