Effects of hyperlipidemias in hamsters on lipid transfer protein activity and unidirectional cholesteryl ester transfer in plasma

• Published in: Biochimica et biophysica acta Vol. 1083; no. 3; pp. 257 - 264
• Main Authors: Quig, David W., Arbeeny, Cynthia M., Zilversmit, Donald B.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 03.06.1991; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Apolipoprotein; Binding and carrier proteins; Biological and medical sciences; Carrier Proteins - antagonists & inhibitors; Carrier Proteins - blood; Cholesterol Ester Transfer Proteins; Cholesterol Esters - blood; Cholesterol, Dietary - metabolism; Cholesterol-feeding; Cricetinae; Fundamental and applied biological sciences. Psychology; Glycoproteins; Hypercholesterolemia; Hyperlipidemias - blood; Hyperlipidemias - metabolism; Hypertriglyceridemia; Lipoproteins - metabolism; Lipoproteins, HDL - blood; Male; Mesocricetus; Proteins; Rabbits; Lipid transfer activity; Plasma d > 1.21 g/ml LTA; Cholesteryl esters; Hypercholesterolemia; Hypertriglyceridemia; Lipid transfer protein; Apolipoprotein; Cholesterol-feeding; Vertebrata; Mammalia; Radiolabelling; Biological transport; Rodentia; Lipids; Hyperlipemia; Hamster; Carrier protein; Blood plasma
• ISSN: 0005-2760; 0006-3002; 1879-145X
• DOI: 10.1016/0005-2760(91)90080-2

Experiments were performed to characterize plasma lipid transfer protein activity (LTA), and the rate of [ 3H]CE transfer from HDL to lower density lipoproteins in plasma of hamsters. Compared to rabbits, hamster plasma has about one-tenth the level of d > 1.21 LTA but a relatively high level of VLDL-triacylglycerols, and a higher fractional rate of HDL-[ 3H]CE transfer in plasma (in vitro) than predicted by the d > 1.21 LTA. Like the rat, hamster plasma contains an inhibitors(s) of LTA; the level of the inhibitor activity in d > 1.21 g/ml plasma was similar in normal and hyperlipoproteinemic hamsters. Hypertriglyceridemia in sucrose-fed hamsters did not affect LTA, cholesteryl ester transfer or the plasma level of HDL-CE. However, a comparable degree of hypercholesterolemia was associated with a 122% increase in plasma d > 1.21 LTA and a 63% increase in the fractional rate of [ 3H]CE transfer from HDL to lower density lipoproteins in plasma. Cholesterol feeding in hamsters was associated with increased plasma levels of LDL-cholesterol and, to a lesser extent, with VLDL- and IDL-cholesterol.