Isolation and Characterization of Antagonistic Bacteria Paenibacillus jamilae HS-26 and Their Effects on Plant Growth

• Published in: BioMed research international Vol. 2019; no. 2019; pp. 1 - 13
• Main Authors: Liu, Xunli, Zhou, Yanyan, Du, Jianfeng, Liu, Zhaoyang, Zhang, Jiamiao, Sui, Jun-Kang, Li, Qian, Wang, Xiaohui, Xu, Ruiping
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2019; Hindawi; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Acid production; Antifungal agents; Bacteria; Biological control; Biomedical research; Crop diseases; Data processing; Disease control; Enzymes; Fertilizers; Fixation; Food plants; Food production; Fungi; Fungicides; Gas chromatography; Growth; In vitro methods and tests; Indoleacetic acid; Mass spectrometry; Mass spectroscopy; Metabolites; Microorganisms; Mycelia; Nitrogen; Nitrogen fixation; Organic chemistry; Organic compounds; Paenibacillus; Pathogens; Pesticides; Pests; Phenylenediamine; Phosphates; Plant diseases; Plant growth; Plant pathology; Potassium; Probiotics; Rhizosphere; Soils; Solubilization; VOCs; Volatile organic compounds; Wheat; China
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2019/3638926

Soilborne pathogens affect plant growth and food production worldwide. The application of chemical fertilizers and pesticides to control plant diseases has harmful effects; fortunately, plant growth-promoting rhizobacteria can be used as a potential alternative strategy. Here, Paenibacillus jamilae HS-26 was selected for its highly antagonistic activity against several soilborne pathogens. The bacterium synthesized hydrolytic enzymes and released extracellular antifungal metabolites and volatile organic compounds—primarily, N, N-diethyl-1, 4-phenylenediamine, which was detected by gas chromatography-mass spectrometry and shown to inhibit fungal mycelial growth. Furthermore, HS-26 was useful for nitrogen fixation, phosphate and potassium solubilization, and siderophore and indoleacetic acid production. In vitro tests and pot experiments revealed that HS-26 considerably increased plant biometric parameters. Illumina MiSeq sequencing data showed a significant reduction in soilborne pathogens and increase in beneficial bacteria in the wheat rhizosphere after treatment with strain HS-26.