Hybrid Sterility in Fish Caused by Mitotic Arrest of Primordial Germ Cells

• Published in: Genetics (Austin) Vol. 209; no. 2; pp. 507 - 521
• Main Authors: Yoshikawa, Hiroyuki, Xu, Dongdong, Ino, Yasuko, Yoshino, Tasuku, Hayashida, Takao, Wang, Junjie, Yazawa, Ryosuke, Yoshizaki, Goro, Takeuchi, Yutaka
• Format: Journal Article
• Language: English
• Published: United States Genetics Society of America 01.06.2018
• Subjects: Animals; Argyrosomus japonicus; Cell migration; Cellular Microenvironment; Chromosomes; Embryos; Female; Fish; Gametes; Gametogenesis; Genetics; Germ cells; Germ Cells - cytology; Germ Cells - transplantation; Gonads; Gonads - cytology; Gonads - growth & development; Homology; Hybridization, Genetic; Hybrids; Infertility - genetics; Infertility - pathology; Investigations; Male; Marine fish; Meiosis; Mitosis; Molecular chains; Nibea albiflora; Nibea mitsukurii; Pennahia argentata; Perciformes - genetics; Perciformes - growth & development; Perciformes - physiology; Phenotypes; Salmon; Sciaenidae; Sterility; Transplantation; Zebrafish; sterility; germline; gametogenesis; sexual reproduction; genetics of sex; primordial germ cells; hybridization; mitosis
• ISSN: 1943-2631; 0016-6731; 1943-2631
• DOI: 10.1534/genetics.118.300777

Sterility in hybrid animals is widely known to be due to a cytological mechanism of aberrant homologous chromosome pairing during meiosis in hybrid germ cells. In this study, the gametes of four marine fish species belonging to the Sciaenid family were artificially fertilized, and germ cell development was examined at the cellular and molecular levels. One of the intergeneric hybrids had gonads that were testis-like in structure, small in size, and lacked germ cells. Specification of primordial germ cells (PGCs) and their migration toward genital ridges occurred normally in hybrid embryos, but these PGCs did not proliferate in the hybrid gonads. By germ cell transplantation assay, we showed that the gonadal microenvironment in hybrid recipients produced functional donor-derived gametes, suggesting that the germ cell-less phenotype was caused by cell autonomous proliferative defects of hybrid PGCs. This is the first evidence of mitotic arrest of germ cells causing hybrid sterility in animals.