Oleuropein-Rich Jasminum Grandiflorum Flower Extract Regulates the LKB1-PGC-1α Axis Related to the Attenuation of Hepatocellular Lipid Dysmetabolism

• Published in: Nutrients Vol. 16; no. 1; p. 58
• Main Authors: Hou, Yajun, Zhao, Xuan, Wang, Yalin, Li, Yapeng, Chen, Caihong, Zhou, Xiu, Jin, Jingwei, Ye, Jiming, Li, Dongli, Gan, Lishe, Wu, Rihui
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24.12.2023; MDPI
• Subjects: Bioengineering; Calories; Chromatography; Dietary supplements; Ethanol; Ethylenediaminetetraacetic acid; Fatty acids; Functional foods; hepatic steatosis; Jasminum grandiflorum; Kinases; Laboratory animals; Liver; Liver diseases; LKB1-PGC-1α axis; Metabolism; Mitochondrial DNA; Monounsaturated fatty acids; Obesity; oleuropein; Protein binding; Protein kinases; Proteins; RNA; Scientific equipment and supplies industry; Synthesis; Type 2 diabetes; United States; Massachusetts; Japan; China; New York; St Louis Missouri; United States > US; oleuropein; Jasminum grandiflorum; LKB1-PGC-1α axis; hepatic steatosis
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu16010058

Diets() rich in fat are a major() cause() of metabolic disease(), and nutritional() food has been widely() used() to counteract the metabolic disorders such() as obesity() and fatty() liver(). The present study investigated the effects of oleuropein-enriched extract() from L. flowers (OLE-JGF) in high-fat diet() (HFD)-fed mice and oleic acid() (OA)-treated AML-12 cells. Treatment() of HFD-fed mice with 0.6% OLE-JGF for 8 weeks significantly reduced body and liver() weights, as well as attenuating lipid dysmetabolism and hepatic steatosis. OLE-JGF administration() prominently suppressed the mRNA expressions() of monocyte chemoattractant protein()-1 (MCP-1) and cluster of differentiation 68 (CD68), and it also downregulated acetyl-CoA carboxylase (ACC) and fatty() acid() synthase (FAS) as well as sterol-regulatory-element()-binding protein() (SREBP-1c) in the liver(). Meanwhile, mitochondrial DNA and uncoupling protein() 2 (UCP2) were upregulated along with the increased expression() of mitochondrial biogenic promoters including liver() kinase B1 (LKB1), peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α), nuclear() factor()-erythroid-derived 2-like 2 (Nrf2), and mitochondrial transcription factor() A (Tfam), but did not change AMP-activated protein() kinase (AMPK) in liver(). The lipid droplets were decreased significantly after treatment() with 80 μM oleuropein for 24 h in OA-induced AML-12 cells. Furthermore, oleuropein significantly inhibited ACC mRNA expression() and upregulated LKB1, PGC-1α, and Tfam mRNA levels, as well as increasing the binding level of LKB1 to PGC-1α promoter in OA-induced cells. These findings indicate() that OLE-JGF reduces hepatic lipid deposition in HFD-fed mice, as well as the fact that OA-induced liver() cells may be partly() attributed to upregulation of the LKB1-PGC-1α axis, which mediates hepatic lipogenesis and mitochondrial biogenesis. Our study provides a scientific() basis() for the benefits and potential() use() of the flower as a food supplement() for the prevention() and treatment() of metabolic disease().