Evaluating physiological responses of plants to salinity stress

• Published in: Annals of botany Vol. 119; no. 1; pp. 1 - 12
• Main Authors: Negrão, S., Schmöckel, S. M., Tester, M.
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.01.2017
• Subjects: Botany - methods; experimental design; genes; guidelines; photosynthesis; Photosynthesis - physiology; physiological response; Plant Development - physiology; Plant Physiological Phenomena; Plant Transpiration - physiology; salinity; salt stress; salt tolerance; Salt Tolerance - physiology; Salt-Tolerant Plants - physiology; soil salinity; Stress, Physiological - physiology; transpiration; VIEWPOINT; Water - metabolism; yield components; osmotic stress; quantifying physiological traits; salt-imposition systems; tolerance indices; salt stress phenotyping; Assessing salinity tolerance; analysing salinity data
• ISSN: 0305-7364; 1095-8290; 1095-8290
• DOI: 10.1093/aob/mcw191

Because soil salinity is a major abiotic constraint affecting crop yield, much research has been conducted to develop plants with improved salinity tolerance. Salinity stress impacts many aspects of a plant's physiology, making it difficult to study in toto Instead, it is more tractable to dissect the plant's response into traits that are hypothesized to be involved in the overall tolerance of the plant to salinity. We discuss how to quantify the impact of salinity on different traits, such as relative growth rate, water relations, transpiration, transpiration use efficiency, ionic relations, photosynthesis, senescence, yield and yield components. We also suggest some guidelines to assist with the selection of appropriate experimental systems, imposition of salinity stress, and obtaining and analysing relevant physiological data using appropriate indices. We illustrate how these indices can be used to identify relationships amongst the proposed traits to identify which traits are the most important contributors to salinity tolerance. Salinity tolerance is complex and involves many genes, but progress has been made in studying the mechanisms underlying a plant's response to salinity. Nevertheless, several previous studies on salinity tolerance could have benefited from improved experimental design. We hope that this paper will provide pertinent information to researchers on performing proficient assays and interpreting results from salinity tolerance experiments.