The Role of PinX1 in Growth Control of Breast Cancer Cells and Its Potential Molecular Mechanism by mRNA and lncRNA Expression Profiles Screening

• Published in: BioMed research international Vol. 2014; no. 2014; pp. 1 - 10
• Main Authors: Zhou, Jue-Yu, Shi, Rong, Zhou, Hui, Zhao, Zhen, Liang, Sang-Hua, Zheng, Wen-Ling, Ma, Wen-Li
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Puplishing Corporation 01.01.2014; Hindawi Publishing Corporation; Hindawi Limited
• Subjects: Breast cancer; Breast Neoplasms - genetics; Breast Neoplasms - pathology; Cell culture; Cell cycle; Cell Line, Tumor; Cell Proliferation; Cloning; DNA methylation; Female; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Gene Knockdown Techniques; Gene Ontology; Genes; Genetic engineering; Growth rate; Humans; Oligonucleotide Array Sequence Analysis; Proteins; Real-Time Polymerase Chain Reaction; Reproducibility of Results; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Rodents; Studies; Telomerase; Transfection; Tumor Suppressor Proteins - genetics; Tumor Suppressor Proteins - metabolism; Beijing China; China
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2014/978984

As a major tumor suppressor gene, the role of PinX1 in breast cancer and its molecular mechanism remain unclear. In this study, overexpression of PinX1 was generated in 3 breast cancer cell lines, and knockdown of PinX1 was performed in a nontumorigenic breast cell line. The regulation of PinX1 on cell proliferation and cell cycle was observed. A microarray-based lncRNA and mRNA expression profile screening was also performed. We found a lower growth rate, G0/G1 phase arrest, and S phase inhibition in the PinX1 overexpressed breast cancer cells, while a higher growth rate, decreased G0/G1 phase, and increased S phase rate in the PinX1 knocked-down nontumorigenic breast cell. A total of 977 mRNAs and 631 lncRNAs were identified as differentially expressed transcripts between PinX1 overexpressed and control MCF-7 cells. Further analysis identified the involvement of these mRNAs in 52 cancer related pathways and various other biological processes. 11 enhancer-like lncRNAs and 25 lincRNAs with their adjacent mRNA pairs were identified as coregulated transcripts. Our results confirmed the role of PinX1 as a major tumor suppressor gene in breast cancer cell lines and provided information for further research on the molecular mechanisms of PinX1 in tumorigenesis.