Characterisation of abamectin resistance in a field-evolved multiresistant population of Plutella xylostella

• Published in: Pest management science Vol. 66; no. 4; pp. 371 - 378
• Main Authors: Pu, Xin, Yang, Yihua, Wu, Shuwen, Wu, Yidong
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.04.2010; Wiley; Wiley Subscription Services, Inc
• Subjects: abamectin; analogs & derivatives; Animals; application rate; Benzoic acid; biochemical mechanism; Biological and medical sciences; Butterflies & moths; China; Control; cross resistance; cytochrome P-450; defense mechanisms; drug effects; Drug Resistance, Multiple; Drug Resistance, Multiple - drug effects; Drug Resistance, Multiple - genetics; Drug Synergism; emamectin benzoate; enzymology; evolution; Evolution, Molecular; Female; Fipronil; Fundamental and applied biological sciences. Psychology; Fungicides; genetics; genotype; incomplete dominance; inheritance; inheritance (genetics); Insect control; insect pests; insecticide resistance; Insecticide Resistance - drug effects; Insecticide Resistance - genetics; Insecticides; Insecticides - pharmacology; Ivermectin; Ivermectin - analogs & derivatives; Ivermectin - pharmacology; Lepidoptera; Lepidoptera - drug effects; Lepidoptera - enzymology; Lepidoptera - genetics; Lepidoptera - physiology; Male; Metabolism; natural selection; oxidation; Pest control; Pesticides; pharmacology; physiology; Phytopathology. Animal pests. Plant and forest protection; plant pests; Plutella xylostella; polygenic inheritance; Protozoa. Invertebrates; resistance; Resistance to control; spinosad; China; Abamectin; biochemical mechanism; Insecta; inheritance; Biochemistry; Genetic determinism; resistance; Plutellidae; Plutella xylostella; Pest; Arthropoda; Multiple resistance; Lepidoptera; Avermectins; Population; Biopesticide; Invertebrata; Microbial insecticide
• ISSN: 1526-498X; 1526-4998; 1526-4998
• DOI: 10.1002/ps.1885

BACKGROUND: Plutella xylostella (L.) has evolved resistance to various kinds of insecticide in the field. Reversion and selection, cross-resistance, inheritance and mechanisms of abamectin resistance were characterised in a field-derived multiresistant population of P. xylostella from China.RESULTS: Compared with a susceptible Roth strain, the field-derived TH population showed ~5000-fold resistance to abamectin. Rapid reversion of abamectin resistance was observed in the TH population when kept without insecticide selection. The TH-Abm strain, selected from the TH population with abamectin, developed 23 670-fold resistance to abamectin, a high level of cross-resistance to emamectin benzoate and low levels of cross-resistance to spinosad and fipronil. Genetic analyses indicated that abamectin resistance in the TH-Abm strain was autosomal, incompletely dominant and polygenic. P450 monooxygenase activities in the TH-Abm strain were significantly elevated compared with the TH strain. Piperonyl butoxide (PBO) inhibited a small part of abamectin resistance in the TH-Abm strain.CONCLUSION: Field-evolved high-level resistance to abamectin in the TH population was not stable. Selection of the TH population with abamectin resulted in an extremely high level of cross-resistance to emamectin benzoate and low levels of cross-resistance to spinosad and fipronil. Enhanced oxidative metabolism was involved in, but may not be the major mechanism of, polygenic abamectin resistance in the TH-Abm strain.