Interleukin-33 could play an important role in the pathogenesis of periodontitis
Background and Objective Interleukin‐33 (IL‐33) controls T‐helper type 2 (Th2) cytokines and the development of mast cells. This study aimed to investigate the expression of IL‐33 and its association with RANKL and osteoprotegerin (OPG) in periodontal health and experimental periodontitis. Material...
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Published in | Journal of periodontal research Vol. 50; no. 4; pp. 525 - 534 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Blackwell Publishing Ltd
01.08.2015
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Subjects | |
Online Access | Get full text |
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Summary: | Background and Objective
Interleukin‐33 (IL‐33) controls T‐helper type 2 (Th2) cytokines and the development of mast cells. This study aimed to investigate the expression of IL‐33 and its association with RANKL and osteoprotegerin (OPG) in periodontal health and experimental periodontitis.
Material and Methods
Eighteen Wistar rats were assigned to two study groups of nine animals each: ligature only (LO) and nonligated (NL). Silk sutures were placed subgingivally, surrounding the right lower first molars. The animals were killed on day 11 after ligature placement, and the alveolar bone loss at the first molars was determined histometrically. Periodontal tissues were examined histopathologically to evaluate the differences between the groups. The expression of IL‐33, RANKL and OPG was detected immunohistochemically.
Results
The LO group showed significantly greater alveolar bone loss compared with the NL group (p < 0.05). The numbers of osteoclasts, osteoblasts and inflammatory cells were significantly higher in the LO group compared with the NL group (p < 0.05). Osteoblastic activity was significantly lower in the LO group than in the NL group (p < 0.05). There was significantly higher expression of IL‐33 and RANKL and a greater number of OPG‐positive cells in the LO group (p < 0.05). IL‐33 expression showed a positive correlation with RANKL expression and with the number of mast cells (p < 0.05).
Conclusion
The experimental periodontitis group exhibited increased expression of IL‐33 and RANKL compared with the healthy group. Additionally, there was a positive correlation between these expressions. According to these results, IL‐33 could be associated with the pathogenesis of periodontal disease. |
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Bibliography: | ark:/67375/WNG-53WGRLW9-6 istex:4E97E2CA06E289A8CAC06163CE88BE7BC6FDF01D ArticleID:JRE12235 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0022-3484 1600-0765 |
DOI: | 10.1111/jre.12235 |