hierarchical analysis of transcriptome alterations in intrauterine growth restriction (IUGR) reveals common pathophysiological pathways in mammals

• Published in: The Journal of pathology Vol. 213; no. 3; pp. 337 - 346
• Main Authors: Buffat, C, Mondon, F, Rigourd, V, Boubred, F, Bessières, B, Fayol, L, Feuerstein, J.M, Gamerre, M, Jammes, H, Rebourcet, R, Miralles, F, Courbières, B, Basire, A, Dignat-Georges, F, Carbonne, B, Simeoni, U, Vaiman, D
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.11.2007; Wiley
• Subjects: Adult; Analysis of Variance; Animals; Biological and medical sciences; Case-Control Studies; Cluster Analysis; Computer Science; Female; Fetal Growth Retardation - genetics; Fetal Growth Retardation - metabolism; Gene Expression Profiling; Humans; Immunohistochemistry; Infant, Newborn; Investigative techniques, diagnostic techniques (general aspects); IUGR; Life Sciences; Medical sciences; Medicin och hälsovetenskap; microarrays; Models, Animal; Oligonucleotide Array Sequence Analysis; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; PDX1; placenta; Placenta - metabolism; Pregnancy; Principal Component Analysis; Promoter Regions, Genetic; Rats; Rats, Sprague-Dawley; Rattus Norvegicus; Reverse Transcriptase Polymerase Chain Reaction; Statistics, Nonparametric; Transcription, Genetic; Pathophysiology; Fetal membrane; transcriptome; Rodentia; Rattus norvegicus; PDX1; In utero; Growth retardation; microarrays; Pregnancy; Vertebrata; Anatomic pathology; Mammalia; Placenta; Uterus; IUGR; Animal; Female genital system; pdx1gene; MICROARRAYS; RAT; RATTUS NORVEGICUS; PLACENTA
• ISSN: 0022-3417; 1096-9896
• DOI: 10.1002/path.2233

Intra-uterine growth restriction (IUGR) is a frequent disease, affecting up to 10% of human pregnancies and responsible for increased perinatal morbidity and mortality. Moreover, low birth weight is an important cause of the metabolic syndrome in the adult. Protein depletion during the gestation of rat females has been widely used as a model for human IUGR. By transcriptome analysis of control and protein-deprived rat placentas, we were able to identify 2543 transcripts modified more than 2.5 fold (1347 induced and 1196 repressed). Automatic functional classification enabled us to identify clusters of induced genes affecting chromosome structure, transcription, intracellular transport, protein modifications and apoptosis. In particular, we suggest the existence of a complex balance regulating apoptosis. Among repressed genes, we noted several groups of genes involved in immunity, signalling and degradation of noxious chemicals. These observations suggest that IUGR placentas have a decreased resistance to external aggression. The promoters of the most induced and most repressed genes were contrasted for their composition in putative transcription factor binding sites. There was an over-representation of Zn finger (ZNF) proteins and Pdx1 (pancreatic and duodenal homeobox protein 1) putative binding sites. Consistently, Pdx1 and a high proportion of ZNF genes were induced at the transcriptional level. A similar analysis of ZNF promoters showed an increased presence of putative binding sites for the Tata box binding protein (Tbp). Consistently again, we showed that the Tbp and TBP-associated factors (Tafs) were up-regulated in IUGR placentas. Also, samples of human IUGR and control placentas showed that human orthologous ZNFs and PDX1 were transcriptionnally induced, especially in non-vascular IUGR. Immunohistochemistry revealed increased expression of PDX1 in IUGR human placentas. In conclusion, our approach permitted the proposition of hypotheses on a hierarchy of gene inductions/repressions leading to massive transcriptional alterations in the IUGR placenta, in humans and in rodents. Copyright © 2007 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.