Silencing of PNPLA6 , the neuropathy target esterase (NTE) codifying gene, alters neurodifferentiation of human embryonal carcinoma stem cells (NT2)

• Published in: Neuroscience Vol. 281; pp. 54 - 67
• Main Authors: Pamies, D, Bal-Price, A, Fabbri, M, Gribaldo, L, Scelfo, B, Harris, G, Collotta, A, Vilanova, E, Sogorb, M.A
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 05.12.2014; Elsevier
• Subjects: Biological and medical sciences; Carboxylic Ester Hydrolases - genetics; Cell Differentiation - genetics; Embryonal Carcinoma Stem Cells; Fundamental and applied biological sciences. Psychology; Gene Expression - genetics; Gene Silencing; hNT2; Humans; in vitro; Medical sciences; Multiple tumors. Solid tumors. Tumors in childhood (general aspects); neurodifferentiation; Neurology; neuropathy target esterase; Phospholipases - genetics; PNPLA6; silencing; Tumors; Vertebrates: nervous system and sense organs; NANOG homeobox; myelocytomatosis oncogene; neurofilament heavy polypeptide; siRNA for PNPLA6; medium polypeptide neurofilament; phosphate-buffered saline; MEAs; silencing; SOX2; NF200; neurodifferentiation; SRY-box-containing gene 2; MAP2; UCN2; FBS; MYC; neurobeachin-like 1; silencing RNAs; ethylenediaminetetraacetic acid; NCAM1; NEFM; PBS; microelectrode arrays; DAPI; 4′,6-diamidino-2-phenylindole; NBEAL1; siRNA; EDTA; siPNPLA6; neuropathy target esterase; GFAP; DIV; fetal bovine serum; hNT2; in vitro; neural cell adhesion molecule 1; patatin-like phospholipase family; days in vitro; NANOG; human-derived NTera2/D1; monoglyceride lipase; PNPLA6; glial fibrillary acidic protein; urocortin 2; microtubule-associated protein 2; MGLL; NTE; Human; Enzyme; Stem cell; Esterases; Malignant tumor; In vitro; Hydrolases; Cancer; Embryonal carcinoma
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2014.08.031

Highlights • PNPLA6 silencing led to gene alterations associated with several developmental processes. • PNPLA6 silencing led to important phenotypic alterations during differentiation in vitro. • PNPLA6 plays a role during the early stages of differentiation.