Salinity induced limitations on photosynthesis in Prunus salicina, a deciduous tree species

• Published in: Plant physiology (Bethesda) Vol. 93; no. 3; pp. 864 - 870
• Main Authors: Ziska, Lewis H., Seemann, Jeffrey R., DeJong, Theodore M.
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.07.1990
• Subjects: acide organique; acidos organicos; actividad enzimatica; activite enzymatique; agua de riego; azucares; Biochemistry; Biological and medical sciences; california; californie; carbohidratos; carbohydrates; Carbon dioxide; Chlorides; chlorophylle; chlorophylls; chlorure; clorofilas; cloruros; composicion; composition; eau d' irrigation; enzymic activity; feuille; fotosintesis; Fundamental and applied biological sciences. Psychology; glucide; hojas; irrigation water; Leaf area; Leaves; liasas; lyase; lyases; Metabolism; organic acids; photosynthese; Photosynthesis; Photosynthesis, respiration. Anabolism, catabolism; Plant physiology and development; Plants; prunus salicina; Respiration; salinidad; salinite; Salinity; Stomatal conductance; stress; sucres; sugars; transpiracion; transpiration; Metabolite; Toxicity; Carbon dioxide; Stomatal conductance; Rosaceae; Plant leaf; Field experiment; Salinity; Dicotyledones; Chlorides; Angiospermae; Spermatophyta; Gas exchange; Biological accumulation; Photosynthesis
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.93.3.864

The response of photosynthetic CO2 assimilation to salinization in 19 year old Prunus salicina was evaluated under field conditions for a 3 year period. The observed decline in CO2 assimilation capacity was apparently related to increasing leaf chloride (Cl-) content, and independent of changes in leaf carbohydrate status. The response of net CO2 assimilation (A) to leaf intercellular CO2 partial pressure (Ci) indicated that the reduction in the capacity for A with Cl- was not the result of decreased stomatal conductance but a consequence of nonstomatal inhibition. The nonstomatal limitations to CO2 assimilation capacity, as determined by the response of A to Ci and biochemical assay, were related to a decline in the activity of ribulose, 1,5-bisphosphate carboxylase (Rubpcase) and the pool size of triose phosphate, ribulose 1,5-bisphosphate (Rubp) and phosphoglycerate with increasing salinity. Lack of agreement between the initial slope of the A to Ci response curve and Rubpcase activity suggests the occurrence of heterogeneous stomatal apertures with the high salinity treatment (28 millimolar). Prolonged exposure to chloride salts appeared to increase the Rubp of Pi regeneration limitation, decrease Rubpcase activity and reduce leaf chlorophyll content. Observed changes in the biochemical components of CO2 fixation may, in turn, affect total leaf carbohydrates, which also declined with time and salinity. The reduction in Rubpcase activity was apparently a consequence of a reduced Rubpcase protein level rather than either a regulatory or inhibitory effect.