Molecular identification of Cordylobia anthropophaga Blanchard (Diptera: Calliphoridae) larvae collected from dogs (Canis familiaris) in Jos South, Plateau State, Nigeria

Myiasis-causing larvae were extracted from dogs attending veterinary clinics in Plateau State, Nigeria and subjected to molecular analysis involving polymerase chain reaction amplification of the 28S rRNA gene of blowflies, cloning and sequencing techniques. All larvae were confirmed as Cordylobia a...

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Published inOnderstepoort journal of veterinary research Vol. 79; no. 1; pp. E1 - E4
Main Authors Ogo, Ndudim I, Onovoh, Emmanuel, Okubanjo, Oluyinka O, Galindo, Ruth C, De la Lastra, Jose-Manuel P, De la Fuente, Jose
Format Journal Article
LanguageEnglish
Portuguese
Published South Africa AOSIS (Pty) Ltd 2012
ARC-Onderstepoort Veterinary Institute - Agricultural Research Council
Subjects
Agriculture, Dairy & Animal Science
Anatomy & Morphology
Animals
Biochemistry & Molecular Biology
Diptera - classification
Diptera - genetics
Dog Diseases - parasitology
Dogs
Gene Amplification
Larva
Myiasis - parasitology
Myiasis - veterinary
Nigeria
Pharmacology & Pharmacy
Phylogeny
Physiology
Polymerase Chain Reaction - veterinary
RNA, Ribosomal, 28S - genetics
Species Specificity
Veterinary Sciences
Nigeria
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Summary:Myiasis-causing larvae were extracted from dogs attending veterinary clinics in Plateau State, Nigeria and subjected to molecular analysis involving polymerase chain reaction amplification of the 28S rRNA gene of blowflies, cloning and sequencing techniques. All larvae were confirmed as Cordylobia anthropophaga Blanchard (Diptera: Calliphoridae) after the initial morphological identification. This is the first molecular identification of any myiasis-causing fly species in Nigeria and may serve as a reliable alternative to morphological identification where samples are not well preserved or difficult to identify to species level.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0030-2465
2219-0635
2219-0635
DOI:10.4102/ojvr.v79i1.349