Production and Characteristics of the Whole-Cell Lipase from Organic Solvent Tolerant Burkholderia sp. ZYB002

• Published in: Applied biochemistry and biotechnology Vol. 166; no. 3; pp. 536 - 548
• Main Authors: Shu, Zheng-Yu, Wu, Ji-Guang, Cheng, Lan-Xing, Chen, De, Jiang, Yong-Mei, Li, Xin, Huang, Jian-Zhong
• Format: Journal Article
• Language: English
• Published: New York Humana Press Inc 01.02.2012; Springer; Springer Nature B.V
• Subjects: 1-Butanol - chemistry; Bacterial Proteins - isolation & purification; Bacterial Proteins - metabolism; Biochemistry; Biological and medical sciences; Biotechnology; Burkholderia; Burkholderia - enzymology; Burkholderia - isolation & purification; Chemistry; Chemistry and Materials Science; Culture Media; Environmental Restoration and Remediation - methods; Enzyme Stability; Enzymes; Fuel Oils; Fundamental and applied biological sciences. Psychology; Hot Temperature; Hydrogen-Ion Concentration; Kinetics; Lipase - isolation & purification; Lipase - metabolism; Manganese; Olea; Olive oil; Soil Microbiology; Soil Pollutants - metabolism; Solvents; Solvents - chemistry; Soybean oil; Soybeans; Substrate Specificity; Surface-Active Agents - chemistry; sp. ZYB002; Organic solvent tolerance; Whole cell lipase; Enzyme characteristics; Production; Burkholderia sp; Enzyme; Tolerance; Triacylglycerol lipase; Esterases; Carboxylic ester hydrolases; Organic solvent; ZYB002; Bacteria; Hydrolases; Burkholderia
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-011-9446-1

The thermostable and organic solvent tolerant whole-cell lipase (WCL) was produced by Burkholderia sp. ZYB002 with broad spectrum organic solvent tolerance. The production medium of the WCL was primarily optimized, which resulted in the maximum activity of 22.8 U/mL and the 5.1-fold increase of the WCL yield. The optimized culture medium was as follows (% w/v or v/v ): soybean meal 2, soybean oil 0.5, manganese sulfate 0.1, K 2 HPO 4 0.1, olive oil 0.5, initial pH 6.0, inoculum density 2, liquid volume 35 mL in 250-mL Erlenmeyer flask, and incubation time 24 h. The biochemical characterization of the WCL from Burkholderia sp. ZYB002 was determined, and the results showed that the optimal pH and temperature for lipolytic activity of the WCL was 8.0 and 65°C, respectively. The WCL was stable at temperature up to 70°C for 1 h and retained 79.2% of its original activity. The WCL was highly stable in the pH range from 3.0 to 8.5 for 6 h. Ca 2+ , K + , Na + , NO 3 − , etc. ions stimulated its lipolytic activity, whereas Zn 2+ ion caused inhibition effect. The WCL was also relatively stable in n -butanol at a final concentration of 50% ( v/v ) for 24 h. However, the WCL was strongly inhibited in Triton X-100 at a final concentration of 10% ( v/v ). The WCL with thermal resistance and organic solvent tolerance showed its great potential in various green industrial chemical processes.