Regulation of matrix metalloproteinase secretion by green tea catechins in a three-dimensional co-culture model of macrophages and gingival fibroblasts

• Published in: Archives of oral biology Vol. 75; pp. 89 - 99
• Main Authors: Morin, Marie-Pierre, Grenier, Daniel
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.03.2017
• Subjects: Advanced Basic Science; Aggregatibacter actinomycetemcomitans; Aggregatibacter actinomycetemcomitans - chemistry; Catechin; Catechin - analogs & derivatives; Catechin - pharmacology; Cell Line; Cell Survival - drug effects; Coculture Techniques - methods; Collagen Type I - chemistry; Dentistry; Fibroblast; Fibroblasts - metabolism; Green tea; Humans; Lipopolysaccharide; Lipopolysaccharides - isolation & purification; Lipopolysaccharides - pharmacology; Macrophage; Macrophages - metabolism; Matrix metalloproteinase; Matrix Metalloproteinase 3 - drug effects; Matrix Metalloproteinase 3 - secretion; Matrix Metalloproteinase 8 - drug effects; Matrix Metalloproteinase 8 - secretion; Matrix Metalloproteinase 9 - drug effects; Matrix Metalloproteinase 9 - secretion; Matrix Metalloproteinases - drug effects; Matrix Metalloproteinases - secretion; Periodontal disease; Periodontitis - drug therapy; Plant Extracts - chemistry; Plant Extracts - pharmacology; Polyphenol; Tea - chemistry; U937 Cells; Aggregatibacter actinomycetemcomitans; Catechin; Periodontal disease; Polyphenol; Lipopolysaccharide; Matrix metalloproteinase; Fibroblast; Green tea; Macrophage
• ISSN: 0003-9969; 1879-1506
• DOI: 10.1016/j.archoralbio.2016.10.035

Highlights • A co-culture model of macrophages and gingival fibroblasts with a 10:1 ratio produces higher amounts of MMP-3, −8, and −9 than that with a 1:10 ratio. • The production of MMPs by the co-culture model is inhibited by a green tea extract as well as EGCG, its major constituent. • Green tea catechins offer a preventive and therapeutic potential against periodontal disease.