Selective loss of AMPA receptors at corticothalamic synapses in the epileptic stargazer mouse

• Published in: Neuroscience Vol. 217; pp. 19 - 31
• Main Authors: Barad, Z, Shevtsova, O, Arbuthnott, G.W, Leitch, B
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 16.08.2012; Elsevier
• Subjects: absence epilepsy; alpha -Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors; AMPA receptors; Animals; Biological and medical sciences; CACNG2 protein; Calcium channels (voltage-gated); Calcium Channels - genetics; Cerebral Cortex - metabolism; Cortex; Cytochemistry; Drug development; Electrophysiological recording; Epilepsy; Epilepsy - genetics; Epilepsy - metabolism; Excitatory Postsynaptic Potentials - physiology; Fundamental and applied biological sciences. Psychology; Glutamic acid receptors; Headache. Facial pains. Syncopes. Epilepsia. Intracranial hypertension. Brain oedema. Cerebral palsy; immunogold; Medical sciences; membrane trafficking; Mice; Mice, Neurologic Mutants; Mutation; Nervous system; Nervous system (semeiology, syndromes); Neural Pathways - metabolism; Neurology; Neurons; Neurons - metabolism; Oscillations; Receptors, AMPA - metabolism; Seizures; stargazin; Synapses; Synapses - metabolism; Thalamus; Thalamus - metabolism; Vertebrates: nervous system and sense organs; Western blot; Western blotting; Western blot; α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor; transmembrane AMPA receptor regulatory protein; WB; TARP; RTN; granule cell; GluA; ethylenediaminetetraacetic acid; stargazin; EPSC; ventral posterior thalamus; GC; excitatory postsynaptic current; thalamus; PSD; absence epilepsy; VDCC; GAERS; post-synaptic density; EDTA; AMPAR; reticular thalamic nucleus; immunogold; Genetic Absence Epilepsy Rats from Strasbourg; voltage-dependent calcium channel; glutamate receptor; VP; AMPA receptors; Nervous system diseases; Epilepsy; Rodentia; Central nervous system; Petit mal; Glutamate receptor; Encephalon; Cerebral disorder; Vertebrata; AMPA receptor; Synapse; Mammalia; Mouse; Animal; Central nervous system disease; Thalamus
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2012.05.011

Highlights ► First in vivo study of AMPAR density at thalamic neuron synapses in stargazer mice. ► Selective loss of AMPARs in inhibitory reticular thalamic neuron synapses in stargazer mutants. ► Number of AMPARs is unchanged at corticothalamic synapses of ventral posterior thalamus. ► The loss of excitation on inhibitory neurons may underlie the epilepsy in these mice.