Integrated Biosensor Systems for Ethanol Analysis

• Published in: Applied biochemistry and biotechnology Vol. 146; no. 1-3; pp. 129 - 136
• Main Authors: Alhadeff, Eliana M., Salgado, Andrea M., Cós, Oriol, Pereira, Nei, Valero, Francisco, Valdman, Belkis
• Format: Journal Article Conference Proceeding
• Language: English
• Published: New York Humana Press Inc 01.03.2008; Springer; Springer Nature B.V
• Subjects: Alcohol Oxidoreductases - chemistry; Biochemistry; Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensors; Biotechnology; Chemistry; Chemistry and Materials Science; Colorimetry - instrumentation; Correlation coefficient; Enzymes; Enzymes, Immobilized - chemistry; Equipment Design; Equipment Failure Analysis; Ethanol; Ethanol - analysis; Ethanol - chemistry; Fermentation; Flow Injection Analysis - instrumentation; Fundamental and applied biological sciences. Psychology; Horseradish Peroxidase; Injection; Methods. Procedures. Technologies; Reproducibility of Results; Sensitivity and Specificity; Systems Integration; Various methods and equipments; Horseradish peroxidase; Sequential injection analysis; Flow injection analysis; Alcohol oxidase; Ethanol; Biosensor; Peroxidases; Enzyme; Integrated system; Peroxidase; Oxidoreductases; Flow injection
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-007-8130-y

Different integrated systems with a bi-enzymatic biosensor, working with two different methods for ethanol detection—flow injection analysis (FIA) or sequential injection analysis (SIA)—were developed and applied for ethanol extracted from gasohol mixtures, as well as for samples of alcoholic beverages and fermentation medium. A detection range of 0.05–1.5 g ethanol/l, with a correlation coefficient of 0.9909, has been reached when using FIA system, working with only one microreactor packed with immobilized alcohol oxidase and injecting free horseradish peroxidase. When using both enzymes, immobilized separately in two microreactors, the detection ranges obtained varied from 0.001 to 0.066 g ethanol/l, without on-line dilution to 0.010–0.047 g ethanol/l when a 1:7,000 dilution ratio was employed, reaching correlation coefficients of 0.9897 and 0.9992, respectively. For the integrated biosensor SIA system with the stop–flow technique, the linear range was 0.005–0.04 g/l, with a correlation coefficient of 0.9922.