Purified polysaccharide from Ginkgo biloba leaves inhibits P-selectin-mediated leucocyte adhesion and inflammation

• Published in: Acta pharmacologica Sinica Vol. 29; no. 4; pp. 499 - 506
• Main Authors: Fei, Rui, Fei, Yu, Zheng, Sheng, Gao, Yan-guang, Sun, Hong-xia, Zeng, Xian-lu
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.04.2008; Nature Publishing Group
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Cell Adhesion - drug effects; CHO Cells; Cricetinae; Cricetulus; Dose-Response Relationship, Drug; Endothelium, Vascular - drug effects; Ginkgo biloba; Ginkgo biloba - immunology; Ginkgo biloba - metabolism; HL-60 Cells; Humans; Immunology; Inflammation - immunology; Inflammation - metabolism; Internal Medicine; Medical Microbiology; Mice; Mice, Inbred BALB C; Neutrophils - drug effects; original-article; P-Selectin - immunology; P-Selectin - metabolism; Pharmacology/Toxicology; Plant Leaves - immunology; Plant Leaves - metabolism; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Umbilical Veins - cytology; Vaccine; 中药学; 消炎作用; 白血球; 银杏多糖; cell adhesion inhibition; polysaccharides of; leaves; inflammation; P-selectin
• ISSN: 1671-4083; 1745-7254
• DOI: 10.1111/j.1745-7254.2008.00765.x

Aim： To investigate the anti-inflammatory mechanism of the polysaccharides of Ginkgo biloba leaves （PGBL） by inhibiting leucocyte adhesion. Methods： The rough PGBL were isolated and purified. The anti-inflammatory effects of purified PGBL （p-PGBL） were assayed by ear edema induced by xylol and the acute perito- nitis model in mice. The effect of p-PGBL on inhibiting the interaction between Pselectin and its ligands was investigated by flow cytometry and flow chamber. Results： p-PGBL could effectively inhibit the acute inflammation in mice and interfere with the adhesion of HL-60 cells, a human leukaemia cell line, or neutrophils to P-selectin in static conditions, as well as the adhesion of neutrophils to Chinese hamster ovary cells expressing human P-selectin and human umbilical vein endothelial cells in flow conditions in a dose-dependant manner. Conclusions： p-PGBL can inhibit the inflammatory process through interfering with the interaction between P-selectin and its ligands.