A Thermophilic Cellulase Complex from Phialophora sp. G5 Showing High Capacity in Cellulose Hydrolysis

• Published in: Applied biochemistry and biotechnology Vol. 166; no. 4; pp. 952 - 960
• Main Authors: Zhao, Junqi, Shi, Pengjun, Bai, Yingguo, Huang, Huoqing, Luo, Huiying, Zhang, Huitu, Xu, Donghao, Wang, Yaru, Yao, Bin
• Format: Journal Article
• Language: English
• Published: New York Humana Press Inc 01.02.2012; Springer; Springer Nature B.V
• Subjects: Biochemistry; Biological and medical sciences; Biotechnology; Carbon sources; Cellulase - isolation & purification; Cellulase - metabolism; Cellulose; Cellulose - metabolism; Chemistry; Chemistry and Materials Science; Corn; Dietary Fiber - metabolism; DNA, Intergenic - genetics; Enzyme Stability; Enzymes; Fundamental and applied biological sciences. Psychology; Fungal Proteins - isolation & purification; Fungal Proteins - metabolism; Fungi; Hot Temperature; Hydrogen-Ion Concentration; Hydrolysis; Industrial Microbiology; Kinetics; Microbiology; Phialophora; Phialophora - enzymology; Phialophora - genetics; Phylogeny; Stover; Triticum aestivum; Waste Products; Wheat bran; Zea mays - metabolism; sp. G5; Thermostability; Cellulase complex; Thermophilic; Phialophora; Enzyme; Cellulose; Thermophily; Complexes; Thermal stability; G5; Fungi; Glycosylases; Hydrolysis; Phialophora sp; Cellulase; Glycosidases; Hydrolases; Fungi Imperfecti
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-011-9483-9

A cellulase-producing mesophilic fungal strain, named G5, was isolated from the acidic wastewater and mud of a tin mine and identified as Phialophora sp. based on the internal transcribed spacer sequence. The volumetric activities and specific activities of cellulase induced by different carbon sources (Avicel, corn cob, wheat bran and corn stover) were compared. The cellulase complex of Phialophora sp. G5 exhibited the optimal activities at 60–65 °C and pH 4.0–5.0, and had good long-term thermostability at 50 °C. Compared with the commercial cellulase (Accellerase 1500, Genencor), the enzyme under study showed 60% and 80% of the capacity to hydrolyze pure cellulose and natural cellulose, respectively. This is the first study to report that a cellulytic enzymes complex from Phialophora genus, and the superior properties of this enzyme complex make strain G5 a potential microbial source to produce cellulase for industrial applications, and the production ability could be improved by mutagenesis.