Dynamic in situ chromosome immobilisation and DNA extraction using localized poly(N-isopropylacrylamide) phase transition

A method of in situ chromosome immobilisation and DNA extraction in a microfluidic polymer chip was presented. Light-induced local heating was used to induce poly(N-isopropylacrylamide) phase transition in order to create a hydrogel and embed a single chromosome such that it was immobilised. This wa...

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Published inBiomicrofluidics Vol. 5; no. 3; pp. 031101 - 031101-4
Main Authors Eriksen, Johan, Thilsted, Anil Haraksingh, Marie, Rodolphe, Lüscher, Christopher James, Nielsen, Lars Bue, Svendsen, Winnie Edith, Szabo, Peter, Kristensen, Anders
Format Journal Article
LanguageEnglish
Published United States American Institute of Physics 01.09.2011
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Brief Communications
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Summary:A method of in situ chromosome immobilisation and DNA extraction in a microfluidic polymer chip was presented. Light-induced local heating was used to induce poly(N-isopropylacrylamide) phase transition in order to create a hydrogel and embed a single chromosome such that it was immobilised. This was achieved with the use of a near-infrared laser focused on an absorption layer integrated in the polymer chip in close proximity to the microchannel. It was possible to proceed to DNA extraction while holding on the chromosome at an arbitrary location by introducing protease K into the microchannel.
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These authors contributed equally to this work.
Electronic mail: rodolphe.marie@dtu.nanotech.dtu.dk.
ISSN:1932-1058
1932-1058
DOI:10.1063/1.3637631