Influence of IL1B polymorphism on CpG island hypermethylation in Helicobacter pylori-infected gastric cancer
Helicobacter pylori infection can induce aberrant CpG island hypermethylation in gastric mucosal epithelial cells. Single nucleotide polymorphisms of proinflammatory cytokine genes encoding for interleukin 1B ( IL1B ), IL6 , and IL8 have been demonstrated to be associated with an increased risk of g...
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Published in | Virchows Archiv : an international journal of pathology Vol. 456; no. 6; pp. 647 - 652 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer-Verlag
01.06.2010
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Helicobacter pylori
infection can induce aberrant CpG island hypermethylation in gastric mucosal epithelial cells. Single nucleotide polymorphisms of proinflammatory cytokine genes encoding for interleukin 1B (
IL1B
),
IL6
, and
IL8
have been demonstrated to be associated with an increased risk of gastric cancer. To identify the influence of host genetic factors in CpG island hypermethylation induced by
H. pylori
infection, we analyzed
H. pylori-
infected chronic gastritis (
n
= 111) and gastric cancer samples (
n
= 78) for the methylation status of eight genes previously shown to be hypermethylated in chronic gastritis and single nucleotide polymorphisms of
IL1B
,
IL6
, and
IL8
. The methylation levels were then compared between different genotypes. Gastric cancers from patients with the
IL1B
-511T/T allele showed significantly higher methylation levels in five genes as compared with gastric cancers from
IL1B
-511 C carriers (
P
< 0.05). An increased level of hypermethylation in association with the
IL1B
-511T/T allele was observed in chronic gastritis samples, but the association was not statistically significant. These findings suggest that the
IL1B
-511T/T allele is associated with enhanced hypermethylation of multiple CpG island loci, which might contribute to an increase in the risk for gastric cancer in individuals with
H. pylori
infection and
IL1B
-511T/T allele. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 0945-6317 1432-2307 |
DOI: | 10.1007/s00428-010-0918-4 |