Fatty acid-binding protein 5 aggravates pulmonary artery fibrosis in pulmonary hypertension secondary to left heart disease via activating wnt/β-catenin pathway

• Published in: Journal of advanced research Vol. 40; pp. 197 - 206
• Main Authors: Lei, Qian, Yu, Zhimin, Li, Hang, Cheng, Jun, Wang, Yanggan
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.09.2022; Elsevier
• Subjects: FABP5; Original; Pulmonary artery fibrosis; Pulmonary hypertension; Pulmonary hypertension secondary to left heart disease; Wnt/β-catenin; Pulmonary hypertension secondary to left heart disease; FABP5; Pulmonary artery fibrosis; Wnt/β-catenin; Pulmonary hypertension
• ISSN: 2090-1232; 2090-1224
• DOI: 10.1016/j.jare.2021.11.011

[Display omitted] •Bioinformatic analysis was performed to screen the key gene of PH-LHD.•FABP5 was identified as a potential target for PH-LHD treatment.•Inhibition of FABP5 improved cardiac function and mitigated pulmonary fibrosis in PH-LHD.•FABP5 upregulated TGF-β1-mediated expression of pro-fibrotic proteins in PAFs.•FABP5 regulated PAF activation partly via activating wnt/β-catenin pathway. Pulmonary hypertension secondary to left heart disease (PH-LHD) is a common and fatal disease. However, no effective therapeutic targets have been identified. Here, we set out to illustrate the functional role and underlying mechanisms of fatty acid-binding protein 5 (FABP5) in PH-LHD development. We performed a systematic analysis of datasets GSE84704 and GSE16624 to identify differentially expressed genes and then constructed protein-protein interaction network for significant modules. Potential target genes in the modules were validated by RT-qPCR and western blot in a PH-LHD mouse model. PH-LHD or sham mice were treated with FABP5 antagonist SBFI-26 or DMSO for 28 days. The role of FABP5 on cardiac function was determined by echocardiography, its impact on pulmonary vascular remodelling were evaluated with right heart catheter, histological analysis and western blot. In vitro, primary pulmonary adventitial fibroblasts were used to investigate the pro-fibrotic mechanisms involving in FABP5. FABP5 was the only one dramatically upregulated along with increased protein expression in the established PH-LHD mouse model. Inhibition of FABP5 by SBFI-26 injection abrogated pulmonary artery remodelling in PH-LHD and improved cardiac function. In vitro, SBFI-26 or FABP5 siRNA blunted the TGF-β1-induced fibrotic response in cultured pulmonary adventitial fibroblasts. Mechanistically, FABP5 knockdown inhibited GSK3β phosphorylation and increased β-catenin phosphorylation. The wnt/β-catenin agonist SKL2001 diminished the antifibrotic effect of FABP5 knockdown on pulmonary adventitial fibroblasts under TGF-β1 stimulation. FABP5 is an important mediator of pulmonary artery remodelling and a potential therapeutic target for PH-LHD.