Isolation, Identification and Optimization of a New Extracellular Lipase Producing Strain of Rhizopus sp

• Published in: Applied biochemistry and biotechnology Vol. 164; no. 7; pp. 969 - 978
• Main Authors: Kantak, Jayshree B., Bagade, Aditi V., Mahajan, Siddharth A., Pawar, Shrikant P., Shouche, Yogesh S., Prabhune, Asmita Ashutosh
• Format: Journal Article
• Language: English
• Published: New York Humana Press Inc 01.08.2011; Springer; Springer Nature B.V
• Subjects: Bacterial Proteins - biosynthesis; Bacterial Proteins - secretion; Biochemistry; Biological and medical sciences; Biotechnology; Biotechnology - methods; Carbon - metabolism; Carbon sources; Chemistry; Chemistry and Materials Science; Culture Media; DNA, Bacterial - analysis; Fermentation; Fundamental and applied biological sciences. Psychology; Fungi; Glucose - metabolism; Hydrogen-Ion Concentration; Kinetics; Lipase - biosynthesis; Lipase - secretion; Liquor; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Nitrogen - metabolism; Optimization techniques; Phylogeny; Rhizopus; Rhizopus - classification; Rhizopus - enzymology; Rhizopus - genetics; Rhizopus - isolation & purification; Rhizopus oryzae; Sequence Analysis, DNA; Soil Microbiology; Soils; Temperature; Zea mays - metabolism; ITS region; Medium optimization; Lipase; Fermentation; Corn steep liquor; Zygomycota; Enzyme; Rhizopus; Corn; Triacylglycerol lipase; Esterases; Identification; Extracellular; Optimization; Carboxylic ester hydrolases; Fungi; Hydrolases; Isolation
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-011-9188-0

A lipolytic mesophilic fungus which produces lipase extracellularly was isolated from soil. Based on ITS1-5.8S–ITS4 region sequences of ribosomal RNA, it was concluded that the isolate JK-1 belongs to genus Rhizopus and clades with Rhizopus oryzae . The present paper reports the screening, isolation, identification, and optimization of fermentation conditions for the production of lipase (EC 3.1.1.3). Culture conditions were optimized, and the highest lipase production was observed in basal medium with corn steep liquor as nitrogen source and glucose as carbon source. Maximum lipase production was observed at 72 h, which is about 870 U/ml. Optimization of fermentation conditions resulted in 16-fold enhancement in enzyme production.