Repercussion of nonsteroidal anti-inflammatory drugs on the gene expression of human osteoblasts

• Published in: PeerJ (San Francisco, CA) Vol. 6; p. e5415
• Main Authors: Melguizo-Rodríguez, Lucia, Costela-Ruiz, Víctor J, Manzano-Moreno, Francisco J, Illescas-Montes, Rebeca, Ramos-Torrecillas, Javier, García-Martínez, Olga, Ruiz, Concepción
• Format: Journal Article
• Language: English
• Published: United States PeerJ, Inc 14.08.2018; PeerJ Inc
• Subjects: Acetaminophen; Analgesics; Bone morphogenetic protein 2; Bone morphogenetic protein 7; Bone tissue; Bones; Cell cycle; Collagen; Diclofenac; Differentiation; Drugs and Devices; Gene expression; Health sciences; Ibuprofen; Indomethacin; Inflammation; Ketoprofen; Laboratories; Mineralization; Naproxen; Nonsteroidal anti-inflammatory drugs; NSAIDs; Osteoblasts; Osteocalcin; Pharmacology; Phosphatase; Physiology; Piroxicam; Systematic review; Toxicology; Transforming growth factor-b1; United States > US; Spain; Bone tissue; Differentiation; Gene expression; Osteoblasts; NSAIDs
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.5415

Nonsteroidal anti-inflammatory drugs (NSAIDs) are frequently used in clinical practice, which can have adverse effects on the osteoblast. The objective of this study was to determine the effect of NSAIDs on the osteoblast by analyzing the gene expression of different markers related to osteoblast maturation and function when treated with different NSAIDs. Three human osteoblast lines from bone samples of three healthy volunteers were treated with 10 µM acetaminophen, indomethacin, ketoprofen, diclofenac, ibuprofen, ketorolac, naproxen, and piroxicam. The gene expression of different markers (run related transcription factor 2 [ ], type 1 collagen [ ], osterix [ ], osteocalcin [ ], bone morphogenetic protein 2 [ ] and 7 [ ], transforming growth factor 1 [ ], and receptors [ ]) were analyzed by real-time PCR at 24 h of treatment. Expression of , was reduced by treatment with all studied NSAIDs, expression was reduced by all NSAIDs except for ketoprofen, naproxen, or piroxicam. Expression of was reduced by all NSAIDs; was reduced by all except for naproxen. In general, NSAID treatment increased the expression of , but not of its receptors ( and ), which was either unchanged or reduced by the treatment. These data confirm that NSAIDs can affect osteoblast physiology, suggesting their possible impact on bone.