Comparative inhibitory activity of the stilbenes resveratrol and oxyresveratrol on African swine fever virus replication

• Published in: Antiviral research Vol. 91; no. 1; pp. 57 - 63
• Main Authors: Galindo, I., Hernáez, B., Berná, J., Fenoll, J., Cenis, J.L., Escribano, J.M., Alonso, C.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 01.07.2011; Elsevier
• Subjects: African swine fever virus; African Swine Fever Virus - drug effects; African Swine Fever Virus - physiology; Animals; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antioxidants - pharmacology; Antiviral; Antiviral agents; Antiviral Agents - pharmacology; Biological and medical sciences; Cell Line; Cercopithecus aethiops; DNA Replication - drug effects; Medical sciences; Oxyresveratrol; Pharmacology. Drug treatments; Phytotherapy; Plant Extracts - pharmacology; Polymerase Chain Reaction; Protein Synthesis Inhibitors - pharmacology; Resveratrol; Stilbenes - pharmacology; Virus Replication - drug effects; Oxyresveratrol; Antiviral; African swine fever virus; Resveratrol; Antioxidant; In vitro; Biological activity; Stilbene derivatives; Virus; Plant origin; Replication; Veterinary; Comparative study
• ISSN: 0166-3542; 1872-9096
• DOI: 10.1016/j.antiviral.2011.04.013

Stilbenols are polyphenolic phytoalexins produced by plants in response to biotic or abiotic stress. These compounds have received much attention because of their significant biological effects. One of these is their antiviral action, which has previously been documented for two members of this class, namely resveratrol and oxyresveratrol. Here we tested the antiviral effect of these two compounds on African swine fever virus, the only member of the newly created family Asfarviridae and a serious limitation to porcine production worldwide. Our results show a potent, dose-dependent antiviral effect of resveratrol and oxyresveratrol in vitro. Interestingly, this antiviral activity was found for these synthetic compounds and also for oxyresveratrol extracted from new natural sources (mulberry twigs). The antiviral effect of these two drugs was demonstrated at concentrations that do not induce cytotoxicity in cultured cells. Moreover, these antivirals achieved a 98–100% reduction in viral titers. Both compounds allowed early protein synthesis but inhibited viral DNA replication, late viral protein synthesis and viral factory formation.