Overexpression of the vesicular acetylcholine transporter increased acetylcholine release in the hippocampus

• Published in: Neuroscience Vol. 218; pp. 1 - 11
• Main Authors: Nagy, P.M, Aubert, I
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 30.08.2012; Elsevier
• Subjects: Acetylcholine; Acetylcholine - metabolism; Acetylcholine - secretion; Acetylcholinesterase; Animals; Biological and medical sciences; Blotting, Western; Brain slice preparation; ChAT; choline acetyltransferase; cholinergic; Cholinergic transmission; Cognitive ability; copy number; eGFP-ChAT; Female; Fundamental and applied biological sciences. Psychology; Gene expression; Hippocampus; Hippocampus - metabolism; Hippocampus - secretion; Immunohistochemistry; Learning; Male; Memory; Mice; Mice, Transgenic; Nervous system; Neurology; Neurotransmission; Reverse Transcriptase Polymerase Chain Reaction; Septum; Synaptic Transmission - physiology; Transgenic mice; VAChT; Vertebrates: nervous system and sense organs; Vesicular Acetylcholine Transport Proteins - metabolism; Vesicular acetylcholine transporter; tris-buffered saline and tween 20; RNA; ACh; Ctx; LV; amygdala nucleus; medial septum; RT-PCR; messenger ribonucleic acid; cDNA; qPCR; CHT1; gDNA; cortex; PBS; BCA; lateral ventricle; MS; qRT-PCR; CPu; thalamic nucleus; TCA; genomic deoxyribonucleic acid; GAPDH; PCR; VAChT; glyceraldehyde 3-phosphate dehydrogenase; trichloroacetic acid; ribonucleic acid; quantitative reverse transcriptase polymerase chain reaction; vesicular acetylcholine transporter; complementary deoxyribonucleic acid; mRNA; bicinchoninic acid; homogenization buffer; relative expression software tool; ChAT; reverse transcriptase polymerase chain reaction; corpus callosum; eGFP-ChAT; crossing point; polymerase chain reaction; cc; knockout; AChE; th nu; eGFP; DAB; KO; 3,3-diaminobenzidine; caudate putamen; quantitative polymerase chain reaction; TBST; acetylcholinesterase; CP; septum; REST; choline acetyltransferase; enhanced green fluorescent protein; high affinity choline transporter; phosphate buffered saline; cholinergic; amyg nu; acetylcholine; HomB; Acyltransferases; Enzyme; Transferases; Central nervous system; Gene overexpression; Choline O-acetyltransferase; Vesicular acetylcholine transporter; Encephalon; Neurotransmitter; Acetylcholine; Hippocampus; Release
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2012.05.047

Highlights ► VAChT genomic DNA is replicated in commercial B6. eGFPChAT mice. ► VAChT mRNA expression was elevated in B6. eGFPChAT septal tissue versus B6 controls. ► VAChT protein was enhanced in the hippocampus and striatum of B6. eGFPChAT mice. ► B6. eGFPChAT mice exhibit enhanced hippocampal acetylcholine release. ► Increasing VAChT expression is sufficient to elevate acetylcholine release.