Differential Antigen Processing by Dendritic Cell Subsets in Vivo

• Published in: Science (American Association for the Advancement of Science) Vol. 315; no. 5808; pp. 107 - 111
• Main Authors: Dudziak, Diana, Kamphorst, Alice O, Heidkamp, Gordon F, Buchholz, Veit R, Trumpfheller, Christine, Yamazaki, Sayuri, Cheong, Cheolho, Liu, Kang, Lee, Han-Woong, Park, Chae Gyu, Steinman, Ralph M, Nussenzweig, Michel C
• Format: Journal Article
• Language: English
• Published: Washington, DC American Association for the Advancement of Science 05.01.2007
• Subjects: Amino Acid Sequence; Animals; Antibodies; Antibodies, Monoclonal - immunology; Antigen Presentation; Antigens; Antigens, CD - analysis; Antigens, CD - immunology; B lymphocytes; Base Sequence; Biological and medical sciences; CD8 Antigens - analysis; CD8 Antigens - immunology; Dendritic cells; Dendritic Cells - immunology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Histocompatibility Antigens Class I - immunology; Histocompatibility Antigens Class II - immunology; Histograms; Lectins, C-Type - analysis; Lectins, C-Type - immunology; Lymphocyte Activation; Mice; Mice, Inbred C3H; Mice, Inbred C57BL; Mice, Transgenic; Minor Histocompatibility Antigens; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; Ova; Receptors, Cell Surface - analysis; Receptors, Cell Surface - immunology; Spleen; T lymphocytes; T-Lymphocytes - immunology; Transgenic animals; Ripening
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1136080

Dendritic cells (DCs) process and present self and foreign antigens to induce tolerance or immunity. In vitro models suggest that induction of immunity is controlled by regulating the presentation of antigen, but little is known about how DCs control antigen presentation in vivo. To examine antigen processing and presentation in vivo, we specifically targeted antigens to two major subsets of DCs by using chimeric monoclonal antibodies. Unlike CD8⁺ DCs that express the cell surface protein CD205, CD8⁻ DCs, which are positive for the 33D1 antigen, are specialized for presentation on major histocompatibility complex (MHC) class II. This difference in antigen processing is intrinsic to the DC subsets and is associated with increased expression of proteins involved in MHC processing.