Epidemics of severe cholera caused by El Tor Vibrio cholerae O1 Ogawa possessing the ctxB gene of the classical biotype in Orissa, India

• Published in: International journal of infectious diseases Vol. 14; no. 5; pp. e384 - e389
• Main Authors: Pal, B.B., Khuntia, H.K., Samal, S.K., Kar, S.K., Patnaik, B.
• Format: Journal Article
• Language: English
• Published: Canada Elsevier Ltd 01.05.2010
• Subjects: Anti-Bacterial Agents - therapeutic use; Cholera - drug therapy; Cholera - epidemiology; Cholera - microbiology; Cholera Toxin - chemistry; Cholera Toxin - genetics; Classical; ctxB gene; Disease Outbreaks; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; El Tor Vibrio cholerae O1; Genotype; Humans; Incidence; India; Microbial Sensitivity Tests; Orissa; Polymerase Chain Reaction; Rural Population; Vibrio cholerae O1 - genetics; Vibrio cholerae O1 - isolation & purification; India; Classical; ctxB gene; Orissa; El Tor Vibrio cholerae O1
• ISSN: 1201-9712; 1878-3511
• DOI: 10.1016/j.ijid.2009.06.020

We investigated the epidemic of cholera that occurred in Kashipur and Dasmantpur blocks of Orissa, reported during July–September 2007. Sixty-two rectal swabs and 28 water samples collected from diarrhea patients at different hospitals and villages were bacteriologically analyzed for the identification, antibiogram, and detection of toxic genes of Vibrio cholerae. The cholera outbreaks were caused by V. cholerae O1 Ogawa biotype El Tor in both Kashipur and Dasmantpur blocks. All the V. cholerae isolates from the clinical and environmental samples were sensitive to tetracycline, gentamicin, azithromycin, and chloramphenicol, but were resistant to ampicillin, ciprofloxacin, norfloxacin, co-trimoxazole, nalidixic acid, neomycin, and furazolidone, except the water isolates, which were sensitive to ciprofloxacin and norfloxacin. The multiplex PCR assay revealed that all the clinical and environmental V. cholerae isolates were positive for the ctxA and tcpA genes, showing biotype El Tor. Interestingly, 88% of the clinical and environmental isolates of V. cholerae were El Tor biotype with mutation at the ctxB gene of the classical strain, as confirmed by mismatch amplification of mutation (MAMA)-PCR assay. This is the first report of the El Tor variant of V. cholerae O1 Ogawa having the ctxB gene of the classical strain with altered antibiogram causing epidemics of cholera in Orissa, India.