Removal of sulfonamide antibiotics by oriented immobilized laccase on Fe3O4 nanoparticles with natural mediators

• Published in: Journal of hazardous materials Vol. 279; pp. 203 - 211
• Main Authors: Shi, Lili, Ma, Fuying, Han, Yuling, Zhang, Xiaoyu, Yu, Hongbo
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 30.08.2014; Elsevier
• Subjects: Affinity; Anti-Bacterial Agents - isolation & purification; Antibiotics; Applied sciences; Atmospheric pollution; Bacteria - drug effects; Binding; Catalysis; Catalytic reactions; Chemical engineering; Chemistry; Covalence; Enzymes, Immobilized - chemistry; Exact sciences and technology; Ferric Compounds - chemistry; General and physical chemistry; Immobilization; Laccase; Laccase - chemistry; Magnetic nanoparticles; Microbial Sensitivity Tests; Models, Molecular; Nanoparticles; Nanoparticles - chemistry; Natural mediator; Pollution; Reactors; Strategy; Sulfonamide antibiotics; Sulfonamides - isolation & purification; Theory of reactions, general kinetics. Catalysis. Nomenclature, chemical documentation, computer chemistry; Transformations; Immobilization; Natural mediator; Sulfonamide antibiotics; Magnetic nanoparticles; Laccase; Catalytic reaction; Stability; Sulfonamide; Nanoparticle; Ultrafine particle; Surface analysis; Antibiotic; Loading; Aerosols; Affinity; Air pollution; Oxidation
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2014.06.070

•Laccase was oriented immobilized on con A-activated Fe3O4 nanoparticles.•The oriented immobilized laccase showed higher removal rate of SAs than free laccase.•Syringic acid mediated almost complete removal of SAs after incubation for 5min.•Laccase mediated covalent cross-coupling of SAs and intermediate of S-type compounds. A novel strategy was applied in the oriented immobilization of laccase from Echinodontium taxodii on concanavalin A-activated Fe3O4 nanoparticles (GAMNs-Con A) based on laccase surface analysis. These nanoparticles showed higher enzyme loading and activity recovery compared with conventional covalent binding. Along with the improvement in thermal and operational stabilities, the oriented immobilized laccase (GAMNs-Con A-L) exhibited higher substrate affinity than free laccase. Free laccase and GAMNs-Con A-L were then applied in the removal of sulfonamide antibiotics (SAs). Although both free and immobilized laccase resulted in the rapid removal of SAs, GAMNs-Con A-L showed a higher removal rate of SAs compared with the free counterpart in the presence of S-type compounds present in lignin structure. Syringic acid mediated the fastest removal efficiency of SAs among S-type compounds and resulted in an almost complete removal of these substances after incubation for 5min. The oxidation products of SAs were identified via LC–ESI+-MS. The results suggested the transformation of SAs and S-type compounds were catalyzed by laccase, resulting in the formation of cross-coupled products.