In silico and in vitro studies on the anti-cancer activity of artemetin, vitexicarpin and penduletin compounds from Vitex negundo

Vitex negundo L. (V. negundo) is one of the important medicinal and anticancer enhancer herbs. This plant is commonly used in the preparation of traditional drugs to treat numerous diseases. Inspired by the medicinal properties of this plant, the current study aimed to investigate antiproliferative...

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Published inSaudi pharmaceutical journal Vol. 30; no. 9; pp. 1301 - 1314
Main Authors Vo, Giau Van, Nguyen, Thi-Hoai-Thu, Nguyen, Thi-Phuong, Do, Thi-Hong-Tuoi, Tran, Nguyen-Minh-An, Nguyen, Huy Truong, Nguyen, Thuy Trang
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.09.2022
Elsevier
Subjects
Anticancer
Artemetin
Docking
HepG2
MCF-7
Original
Penduletin
Vitex negundo
Vitexicarpin
Vitex negundo
MCF-7
HepG2
Docking
Penduletin
Anticancer
Artemetin
Vitexicarpin
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Summary:Vitex negundo L. (V. negundo) is one of the important medicinal and anticancer enhancer herbs. This plant is commonly used in the preparation of traditional drugs to treat numerous diseases. Inspired by the medicinal properties of this plant, the current study aimed to investigate antiproliferative potential and the primary molecular mechanisms of the apoptotic induction against human HepG2 and MCF-7 cell lines, by pure compounds isolated from targeted fractions of V. negundo which were characterized by NMR, FTIR and HRMS analysis and identified as artemetin (FLV1), vitexicarpin (FLV2), and penduletin (FLV3) compounds. The FLV1, FLV2, and FLV3 compounds were evaluated for the antiproliferative potential against HepG2 and MCF-7 cell lines by cell viability assay and exhibited IC50 values of 2.3, 23.9 and 5.6 µM and 3.9, 25.8, and 6.4 µM, respectively. In addition, those compounds increased the level of reactive oxygen species production, induced cell death occurred via apoptosis, demonstrated by Annexin V-staining cells, contributed significantly to DNA damage, and led to the activation of caspase3/caspase8 pathways.Additionally, molecular docking was also conducted to rationalize the cancer cells inhibitory and to evaluate the ability of the FLV1, FLV2, and FLV3 compounds to be developed as good drug candidates for cancers treatment.
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ISSN:1319-0164
2213-7475
DOI:10.1016/j.jsps.2022.06.018