The Effects of Surfactants on the Estimation of Bacterial Density in Petroleum Samples

• Published in: Applied biochemistry and biotechnology Vol. 147; no. 1-3; pp. 77 - 84
• Main Authors: Luna, Aderval Severino, da Costa, Antonio Carlos Augusto, Gonçalves, Márcia Monteiro Machado, de Almeida, Kelly Yaeko Miyashiro
• Format: Journal Article Conference Proceeding
• Language: English
• Published: New York Humana Press Inc 01.03.2008; Springer; Springer Nature B.V
• Subjects: Ammonium; Anaerobic bacteria; Bacteria; Bacteria - isolation & purification; Biochemistry; Biological and medical sciences; Biotechnology; Chemistry; Chemistry and Materials Science; Colony Count, Microbial - methods; Effects; Fundamental and applied biological sciences. Psychology; Petroleum; Petroleum - microbiology; Reproducibility of Results; Sensitivity and Specificity; Studies; Sulfate reduction; Sulfates; Surface-Active Agents - chemistry; Surfactants; Sulphate-reducing bacteria (SRB); Most probable number (MPN); Surfactants; General anaerobic bacteria (GAnB); Petroleum; Sulphate-reducing bacteria (SRB) . General anaerobic bacteria (GAnB); Anaerobe; Sample; Bacteria; Surfactant; Sulfates; Density
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-007-8094-y

The effect of the surfactants polyoxyethylene monostearate (Tween 60), polyoxyethylene monooleate (Tween 80), cetyl trimethyl ammonium bromide (CTAB), and sodium dodecyl sulfate (SDS) on the estimation of bacterial density (sulfate-reducing bacteria [SRB] and general anaerobic bacteria [GAnB]) was examined in petroleum samples. Three different compositions of oil and water were selected to be representative of the real samples. The first one contained a high content of oil, the second one contained a medium content of oil, and the last one contained a low content of oil. The most probable number (MPN) was used to estimate the bacterial density. The results showed that the addition of surfactants did not improve the SRB quantification for the high or medium oil content in the petroleum samples. On other hand, Tween 60 and Tween 80 promoted a significant increase on the GAnB quantification at 0.01% or 0.03% m / v concentrations, respectively. CTAB increased SRB and GAnB estimation for the sample with a low oil content at 0.00005% and 0.0001% m / v , respectively.