Efficiency of graft-transmitted JcFT for floral induction in woody perennial species of the Jatropha genus depends on transport distance

• Published in: Tree physiology Vol. 42; no. 1; pp. 189 - 201
• Main Authors: Tang, Mingyong, Bai, Xue, Wang, Jingxian, Chen, Tao, Meng, Xin, Deng, Hongjun, Li, Chaoqiong, Xu, Zeng-Fu
• Format: Journal Article
• Language: English
• Published: Canada Oxford University Press 05.01.2022
• Subjects: Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Flowers - genetics; Flowers - metabolism; Gene Expression Regulation, Plant; Jatropha - genetics; Jatropha - metabolism; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; Research Paper; florigen transport; Euphorbiaceae; RNA interference; grafting; FLOWERING LOCUS T; Jatropha curcas
• ISSN: 1758-4469; 0829-318X; 1758-4469
• DOI: 10.1093/treephys/tpab116

Abstract FLOWERING LOCUS T (FT) promotes flowering by integrating six genetic pathways. In Arabidopsis, the FT protein is transported from leaves to shoot apices and induces flowering. However, contradictory conclusions about floral induction via graft-transmitted FT in trees were reported in previous studies. We obtained extremely early-flowering transgenic woody Jatropha curcas L. by overexpression of J. curcas FT using Arabidopsis thaliana SUCROSE TRANSPORTER 2 (SUC2) promoter (SUC2:JcFT) and non-flowering transgenic J. curcas by RNA interference (RNAi), which were used to investigate the function of graft-transmitted JcFT in floral induction in woody perennials. Scions from five wild-type species of the Jatropha genus and from JcFT-RNAi transgenic J. curcas were grafted onto SUC2:JcFT rootstocks. Most grafted plants produced flowers in 1–2 months, and the flowering percentage and frequency of various grafted plants decreased with increasing scion length. Consistently, FT protein abundance in scions also decreased with increasing distance from graft junctions to the buds. These findings suggest that FT proteins can be transmitted by grafting and can induce the floral transition in woody perennials, and the efficiency of graft-transmitted JcFT for floral induction depends on the scion length, which may help explain previous seemingly contradictory observations regarding floral induction via graft-transmitted FT in trees. Jatropha curcas florigens (JcFT proteins) can be transmitted by grafting to induce floral transition in woody perennial species of the Jatropha genus, and the efficiency of graft-transmitted JcFT for floral induction depends on the transporting distance.