Antileishmanial activity of furoquinolines and coumarins from Helietta apiculata
The bark infusion of H. apiculata are used to treat wound healing related to cutaneous leishmaniasis and as anti-inflammatory. To isolate, purify active constituents of H. apiculata stem bark, and evaluate their in vitro and in vivo antileishmanial activities. Isolation by chromatographic methods an...
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Published in | Phytomedicine (Stuttgart) Vol. 17; no. 5; pp. 375 - 378 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Germany
Elsevier GmbH
01.04.2010
Urban & Fischer Verlag |
Subjects | |
Online Access | Get full text |
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Summary: | The bark infusion of
H. apiculata are used to treat wound healing related to cutaneous leishmaniasis and as anti-inflammatory.
To isolate, purify active constituents of
H. apiculata stem bark, and evaluate their
in vitro and
in vivo antileishmanial activities.
Isolation by chromatographic methods and chemical identification of furoquinoline alkaloids and coumarins, then evaluation of the
in vitro leishmanicidal activity of these compounds against three strains of
Leishmania sp. promastigotes and
in vivo against
Leishmania amazonensis in Balb/c mice.
Furoquinoline alkaloids and coumarins presented a moderate
in vitro activity against promastigote forms of
Leishmania sp. with IC
50 values in the range between 17 and >50
μg/ml. Balb/c mice infected with
Leishmania amazonensis were treated with γ-fagarine by oral route, or with 3-(1’-dimethylallyl)-decursinol or (-)-heliettin by subscutaneous route for 14 days at 10
mg/kg daily. In these conditions, γ-fagarine, 3-(1’-dimethylallyl)-decursinol and (-)-heliettin showed the same efficacy as the reference drug reducing by 97.4, 95.6 and 98.6% the parasite loads in the lesion, respectively.
These compounds showed significant efficacy in
L. amazonensis infected mice, providing important knowledge to improve its potential role for a future use in the treatment of cutaneous leishmaniasis. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 0944-7113 1618-095X |
DOI: | 10.1016/j.phymed.2009.09.009 |