Low dose of lipopolysaccharide pretreatment can alleviate the inflammatory response in wound infection mouse model

• Published in: Chinese journal of traumatology Vol. 19; no. 4; pp. 193 - 198
• Main Authors: Wang, Dong, Liu, Yang, Zhao, Yan-Rui, Zhou, Jun-Lin
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.08.2016; Department of Orthopedics, Beijing Chao-Yang Hospital, Beijing 100020, China; Elsevier
• Subjects: Animals; Disease Models, Animal; Female; Inflammation - prevention & control; Inflammatory reaction; Lipopolysaccharides; Lipopolysaccharides - therapeutic use; Mice; Mice, Inbred BALB C; Rats; Rats, Wistar; Surgical wound infection; Surgical Wound Infection - drug therapy; Toll-Like Receptor 4 - physiology; Surgical wound infection; Inflammatory reaction; Lipopolysaccharides
• ISSN: 1008-1275
• DOI: 10.1016/j.cjtee.2016.06.001

Purpose： To assess the effects of lipopolysaccharide （LPS） pretreatment on wound infection mouse model and evaluate the biological safety of the optimal pretreatment dose in vivo. Methods： Mice were pretreated with LPS of different doses at 48 and 24 h before femoral medial lon- gitudinal incision was made and infected with different bacteria. Results： It is showed that 0.5 mg/kg/time ofLPS pretreatment can significantly alleviate the inflammation in mouse model infected with methicillin-resistances Staphylococcus aureus, methicillin-sensitive S. aureus, Pseudomonas aeruginosa, or Escherichia coil compared with doses of 0.25 mg/kg/time, 1 mg/ kg/time, and 1.5 mg/kg/time. Conclusions： LPS pretreatment can alleviate the inflammation in mouse model and the optimal dose is 0.5 mg/kg/time, and meanwhile it does not damage organs＇ function.