Protocol of living cell separation using the microfluidic dielectrophoresis integrated chip

• Published in: STAR protocols Vol. 3; no. 3; p. 101527
• Main Authors: Koba, Kazumi, Yarimizu, Kyoko, Fujiyoshi, So, Oshiro, Kyoichi, Wakizaka, Yoshikazu, Takano, Masayo, Maruyama, Fumito
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 16.09.2022; Elsevier
• Subjects: Biotechnology and bioengineering; Cell Biology; Cell isolation; Cell separation/fractionation; Protocol; Cell separation/fractionation; Biotechnology and bioengineering; Cell isolation; Cell Biology
• ISSN: 2666-1667; 2666-1667
• DOI: 10.1016/j.xpro.2022.101527

This protocol demonstrates the separation of living cells with the microfluidic dielectrophoresis chip, using the Jurkat cell as a model. The successful living cell separation lies in familiarity with the detailed tips, which are aided by this stepwise protocol. The knowledge of correct chip installation, sample and buffer filling, flow rate and cell concentration adjustments, and contamination sources increases the efficiency of target viable cell collection. Such instructions, although trivial, are critical for achieving cell separation. For complete details on the use and execution of this protocol, please refer to Oshiro et al. (2022). [Display omitted] •Protocol for living cell separation without using biochemical markers•All-in-one microfluid dielectrophoresis integrated chip•A lab-on-chip cell sorting in 30 min with minimum cell damage•Buffer exchange on the chip: no need for sample pretreatment Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. This protocol demonstrates the separation of living cells with the microfluidic dielectrophoresis chip, using the Jurkat cell as a model. The successful living cell separation lies in familiarity with the detailed tips, which are aided by this stepwise protocol. The knowledge of correct chip installation, sample and buffer filling, flow rate and cell concentration adjustments, and contamination sources increases the efficiency of target viable cell collection. Such instructions, although trivial, are critical for achieving cell separation.