Optical imaging and manipulation of sleeping-brain dynamics in memory processing

• Published in: Neuroscience research Vol. 181; pp. 9 - 16
• Main Author: Miyamoto, Daisuke
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 01.08.2022
• Subjects: Closed-loop optogenetic manipulation; In vivo imaging; Interregional circuits; Learning & memory; Memory replay; Oscillation; Sleep; Synaptic plasticity; Closed-loop optogenetic manipulation; Sleep; Interregional circuits; Memory replay; Oscillation; Synaptic plasticity; Learning & memory; In vivo imaging
• ISSN: 0168-0102; 1872-8111
• DOI: 10.1016/j.neures.2022.04.005

During sleep, the brain undergoes transitions of activity states and reorganization of neural circuits. Recent in vivo optical imaging and manipulation techniques are revealing spatiotemporal and multiscale brain dynamics during sleep and their functional roles in awake state cognition, including learning and memory. First, along with electrophysiological recordings, Ca2+ imaging is becoming the gold standard for tracking the activity of large neural ensembles to analyze memory replay during the sleep/wake cycle in freely moving and head-fixed animals. Comparable to the speed of electrophysiological recordings, voltage indicators can monitor neural activity at millisecond resolution. While one-photon systems have advantages in temporal resolution, two-photon lasers can image microstructures such as dendritic spines and axonal terminals in vivo. Also, optogenetic manipulation is used to perform loss- or gain-of-function experiments with temporal precision, cell-type, and pathway specificity without the artifacts observed with electrophysiological stimulation. In particular, closed-loop optogenetic manipulation is well suited to reveal the functional roles of neural activity and synaptic plasticity in each sleep/wake state. Here, I describe the recent advances in optical imaging and manipulation approaches for sleeping-brain dynamics in memory processing. •Neural activity and synaptic plasticity during sleep contribute to memory processing.•Closed-loop optogenetic manipulation shows functional roles of each sleep/wake state.•Optical imaging illuminates multiscale brain dynamics across the sleep/wake cycle.•Top-down cortical inputs reactivate and consolidate memory during sleep.•Cellular and synaptic engrams are protected during sleep from downregulation.