lncRNA SNHG10 Promotes the Proliferation and Invasion of Osteosarcoma via Wnt/β-Catenin Signaling

• Published in: Molecular therapy. Nucleic acids Vol. 22; pp. 957 - 970
• Main Authors: Zhu, Shutao, Liu, Yang, Wang, Xiao, Wang, Junyi, Xi, Guanghui
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 04.12.2020; American Society of Gene & Cell Therapy; Elsevier
• Subjects: EMT; lncRNA; Original; osteosarcoma; SNHG10; Wnt/β-catenin; lncRNA; EMT; SNHG10; Wnt/β-catenin; osteosarcoma
• ISSN: 2162-2531; 2162-2531
• DOI: 10.1016/j.omtn.2020.10.010

Uncontrolled growth and an enforced epithelial-mesenchymal transition (EMT) process contribute to the poor survival rate of patients with osteosarcoma (OS). Long noncoding RNAs (lncRNAs) have been reported to be involved in the development of OS. However, the significant role of lncRNA SNHG1O on regulating proliferation and the EMT process of OS cells remains unclear. In this study, quantitative real-time PCR and fluorescence in situ hybridization (FISH) results suggested that SNHG10 levels were significantly increased in OS compared with healthy tissues. In vitro experiments (including colony formation, CCK-8, wound healing, and transwell assays) and in vivo experiments indicated that downregulation of SNHG10 significantly suppressed the proliferation and invasion of OS cells. Luciferase reporter assay and RNA immunoprecipitation (RIP) assay confirmed that SNHG10 could regulate FZD3 levels through sponging microRNA 182-5p (miR-182-5p). In addition, the SNHG10/miR-182-5p/FZD3 axis could further promote the β-catenin transfer into nuclear accumulation to maintain the activation of the Wnt singling pathway. Together, our results established that SNHG10 has an important role in promoting OS growth and invasion. By sponging miR-182-5p, SNHG10 can increase FZD3 expression and further maintain the activation of Wnt/β-catenin singling pathway in OS cells. [Display omitted] Zhu et al. demonstrate that overexpressed lncRNA SNHG10 promotes proliferation, migration, and invasion of OS cells by sponging miR-182-5p. The SNHG10/miR-182-5p axis activates the Wnt/β-catenin pathway via elevating FZD3. Targeting SNHG10 may improve the prognosis of patients with OS.