Painting a specific chromosome with CRISPR/Cas9 for live-cell imaging

Dear Editor, Visualization of chromosome shapes and dynamics in a live cell is highly desirable and necessary in many areas of cell biology. For example, the copy number of a particular chromosome in cancer cells is often abnormal （e.g., more than two）, and therefore probing chromosome copy numbers...

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Published inCell research Vol. 27; no. 2; pp. 298 - 301
Main Authors Zhou, Yuexin, Wang, Ping, Tian, Feng, Gao, Ge, Huang, Lei, Wei, Wensheng, Xie, X Sunney
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 01.02.2017
Nature Publishing Group
Subjects
631/1647/1513/1967/3196
631/1647/245/2225
631/80/103
Biomedical and Life Sciences
Cell Biology
Cell Survival
Chromosome Painting
Chromosomes, Human, Pair 9 - genetics
CRISPR-Associated Protein 9 - metabolism
CRISPR-Cas Systems - genetics
HeLa Cells
Humans
Imaging, Three-Dimensional
letter-to-the-editor
Letters to the Editor
Life Sciences
RNA, Guide, CRISPR-Cas Systems - metabolism
动态可视化
拷贝数
染色体
活细胞成像
癌细胞
细胞生物学
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Summary:Dear Editor, Visualization of chromosome shapes and dynamics in a live cell is highly desirable and necessary in many areas of cell biology. For example, the copy number of a particular chromosome in cancer cells is often abnormal （e.g., more than two）, and therefore probing chromosome copy numbers can aid cancer diagnosis.
Bibliography:Dear Editor, Visualization of chromosome shapes and dynamics in a live cell is highly desirable and necessary in many areas of cell biology. For example, the copy number of a particular chromosome in cancer cells is often abnormal （e.g., more than two）, and therefore probing chromosome copy numbers can aid cancer diagnosis.
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These three authors contributed equally to this work.
ISSN:1001-0602
1748-7838
DOI:10.1038/cr.2017.9